外科理论与实践2012，Vol.17Issue(3)：270-274,5.DOI:10.3969/j.issn.1007-1096.2012.03.017
应用猪小肠黏膜下层与肌腱细胞构建组织工程支架修复大鼠腹壁缺损的实验研究
Repair of abdominal wall defect with engineered scaffold using tenocytes seeded porcine small intestinal submucosa in rats
摘要
Abstract
Objective To investigate the biomechanicai properties of the engineered scaffold using tenocytes seeded small intestinal submucosa (SIS) in abdominal wall defect. Methods Abdominal wall defect was repaired using engineered scaffold in rat modes. The rats were sacrificed after 4 weeks and the sample tissues were analyzed by gross observation, histological method and mechanical testing. Results All rats had no abdominal dehiscence or hernia. The engineered scaffold caused slight adhesion with organs. HE and Masson staining proved the junction between SIS and surrounding tissue had more new vessels. The muscle grew into the engineered scaffold. The mechanical strength of the engineered scaffold was much more than the abdominal wall muscles, which could provide sufficient mechanical strength. Conclusions Engineered scaffold repairing abdominal wall defect is a feasible and effective method.关键词
腹壁缺损/肌腱细胞/小肠黏膜下层/组织工程Key words
Abdominal wall defect/ Tenocyte/ Small intestinal submucosa/ Tissue engineering分类
医药卫生引用本文复制引用
宋致成,顾岩..应用猪小肠黏膜下层与肌腱细胞构建组织工程支架修复大鼠腹壁缺损的实验研究[J].外科理论与实践,2012,17(3):270-274,5.基金项目
上海市级医院适宜技术联合开发推广应用项目(SHDC12010204) （SHDC12010204）
上海交通大学医工(理)交叉基金面上项目(YG2009MS36、YG2010MS550) （理）
