西北国防医学杂志2012，Vol.33Issue(2)：110-113,4.
5-脱氧杂氮胞苷调控p16基因表达对人宫颈癌HeLa细胞增殖及调亡的影响
Effects of 5-Aza-CdR on proliferation and apoptosis by modulating p16 gene expression in human cervical carcinoma cell line HeLa
于月成 1吴维光2
作者信息
- 1. 第四军医大学附属西京医院妇产科,陕西西安710032
- 2. 武警医学院附属医院
- 折叠
摘要
Abstract
Objective: To investigate the effects of 5 - aza - 2 - deoxycytidine (5 - Aza - CdR ) on proliferation and apoptosis in human cervical carcinoma cell line HeLa and the possible mechanism. Methods; HeLa cells was treated with different concentration of DNA methytransferase inhibitor 5 - Aza - CdR. Methylation - specific poly-merase chain reaction (MSP) was used to detect promoter methylation status of pl6 gene and RT - PCR was used to detect re - expression of mRNA. Cell growth was estimated by MTT assay and cell apoptosis rate was determined by flow cytometry. Results; Promoter hypermethylation of the pl6 gene was detected in HeLa cells. After treatment with 5 - Aza - CdR, the promoter region of the pl6 gene exhibited a demethylation status, and the p16 gene mRNA was re - expressed. The cellular growth activity decreased and cell apoptosis increased. Conclusion: The 5 - Aza -CdR could reverse the methylation of pl6 gene to regulate the expression of pl6 gene, and could effectively inhibit the cellular proliferation and induce apoptosis of cervical carcinoma cells.关键词
宫颈肿瘤/5-脱氧杂氮胞苷/p16基因/甲基化Key words
Cervix neoplasms/ 5-Aza-CdR/ pl6 gene/ Methylation分类
医药卫生引用本文复制引用
于月成,吴维光..5-脱氧杂氮胞苷调控p16基因表达对人宫颈癌HeLa细胞增殖及调亡的影响[J].西北国防医学杂志,2012,33(2):110-113,4.
