西北国防医学杂志2012，Vol.33Issue(3)：210-213,4.
高原缺氧环境下重症急性胰腺炎合并肠损伤的实验研究
Severe acute pancreatitis complicated with intestinal injury in plateau hypoxia
摘要
Abstract
Objective: To observe the intestinal mucosal tissue of rats with severe acute pancreatitis (SAP) in platuau hypoxia , and investigate the mechanism of gut barrier injury of rats with SAP at high altitude ( H - SAP). Methods: Forty - eight Wistar rats were randomly divided into 2 groups ( n = 24 for each group) , Xi an group ( X) and Maxianshan group (M) according to different altitude. Each group was further divided into four subgroups (n = 6 for each subgroup) , sham - operation group (SO group) , SAP -6 h group, SAP - 12 h group and SAP - 24 h group, 4% sodium taurocholate was injected into the pancreatic duct to induce SAP. Pancreas and intestine tissues were harvested to observe the pathological changes, and the content of TNF - α and IL - 10 in intestine were measured respectively. The ultrastructure of intestine mucosa was observed under transmission electron microscope (TEM). Results;The expression of TNF - α and IL -10 in X - SAP group was significantly different with that in M - SAP group (P < 0.05 ). Under the light microscopy, injury of pancreas and intestine at different time point in M - SAP group was more obvious than that in X - SAP group ( P < 0.05 ). The pathological scores of intestine were positively correlated with that of pancreas in SAP group ( r = 0. 902, P < 0. 01). Injury of intestinal mucosal was more obvious in M - SAP group than that in X - SAP group at each time point under TEM. Conclusion; Injury of intestinal mucosal barrier in rats with SAP can be induced and aggravated in plateau hypoxia. Disturbance of TNF - α and IL - 10 expression play an important role in the pathogenesis and development of intestinal mucosal barrier injury in rats with H - SAP.关键词
重症急性胰腺炎/高原缺氧/肠黏膜屏障Key words
Severe acute pancreatitis/ Plateau hypoxia/ Intestinal mucosal barrier分类
医药卫生引用本文复制引用
张盼,张方信,邓芝云,陈嘉屿,单体栋..高原缺氧环境下重症急性胰腺炎合并肠损伤的实验研究[J].西北国防医学杂志,2012,33(3):210-213,4.基金项目
甘肃省自然科学基金资助项目(1010RJZA052) （1010RJZA052）
