中国组织工程研究2012，Vol.16Issue(27)：4959-4962,4.DOI:10.3969/j.issn.2095-4344.2012.27.004
兔骨髓间充质干细胞与羊膜的共培养
Co-culture of rabbit bone marrow mesenchymal stem cells and human amniotic membrane
摘要
Abstract
BACKGROUND: In recent years, great progress has been made in repair of cartilage defects using tissue engineering technique. Bone marrow mesenchymal stem cells (BMSCs) as common seed cells have been widely used in the tissue engineering of cartilage tissue.
OBJECTIVE: Human amniotic membrane was used as cell scaffold to load rabbit BMSCs and the biocompatibility of human amniotic membrane was observed.
METHODS: Rabbit BMSCs were inoculated onto human acellular amnion. After co-culture, cell morphology was observed. The proliferation of BMSCs was investigated by MTT assay, and the histocompatibility of human amniotic membrane was evaluated. Cell survival and apoptosis was determined by 4', 6-diamidino-2-phenylindole.
RESULTS AND CONCLUTION: BMSCs grew well on the surface of human amniotic membrane and cell viability increased. DAPI staining results showed that only a small number of cells were apoptotic. These findings suggest that human amniotic membrane is a scaffold material with good biocompatibility and can promote the proliferation of BMSCs.关键词
羊膜/骨髓间充质干细胞/生物相容性/兔/细胞支架/干细胞分类
医药卫生引用本文复制引用
张治金,郭林,赵德伟..兔骨髓间充质干细胞与羊膜的共培养[J].中国组织工程研究,2012,16(27):4959-4962,4.
