中国组织工程研究2012，Vol.16Issue(27)：4985-4990,6.DOI:10.3969/j.issn.2095-4344.2012.27.009
成骨诱导脂肪基质细胞在骨组织工程体内成骨中的作用
Osteogenic induction of adipose derived stromal cells in bone tissue engineering in vivo
李晓宇 1姚金凤 2刘政华 1蔡剑波1
作者信息
- 1. 深圳市宝安区人民医院口腔颌面外科,广东省深圳市,518101
- 2. 深圳市第二人民医院口腔科,广东省深圳市,518035
- 折叠
摘要
Abstract
BACKGROUND: Osteogenic induction has been regarded as an indispensable step for adipose-derived stromal cells (ADSCs) to transform into osteoblasts, thus to secret bone matrix to repair bone defects. However, this conclusion has not been confirmed.
OBJECTIVE: Tissue-engineered constructs were prepared from osteogenically induced ADSCs and scaffolds. The constructs were implanted into bone defects and non-bone areas. Whether osteogenically induced ADSCs were transformed into osteoblasts was determined according to bone formation.
METHODS: ADSCs were prepared by collagenase I digestion of subcutaneous fat from the back of dogs aged 12 months. Passage 3 ADSCs were harvested and seeded on the biphasic calcium phosphate ceramics to form a cell-scaffold construct. Box-like bone defects, 20 mm in length and 10 mm in height, were created in the mandibular bilaterally in each dog along the alveolar bone where teeth had been extracted. Then the cell-scaffold constructs were implanted in dog mandibular bone defects. An empty defect group was used as control. The tissue-engineered constructs and osteoinductive calcium phosphate ceramics were implanted in muscle. After implantation for 6 and 12 weeks, bone formation was analyzed using histomorphometry.关键词
脂肪基质细胞/双相磷酸钙陶瓷/成骨诱导/骨缺损/组织工程/干细胞分类
医药卫生引用本文复制引用
李晓宇,姚金凤,刘政华,蔡剑波..成骨诱导脂肪基质细胞在骨组织工程体内成骨中的作用[J].中国组织工程研究,2012,16(27):4985-4990,6.
