中国组织工程研究2012，Vol.16Issue(27)：5072-5075,4.DOI:10.3969/j.issn.2095-4344.2012.27.025
经鼻腔长期输送骨髓基质细胞改善大鼠缺血再灌注性脑损伤
Long term intranasal delivery of bone marrow stromal cells improves rat brain injury caused by ischemia reperfusion
摘要
Abstract
BACKGROUND: Nasal delivery is a highly effective way to deliver drug into the brain, which can overcome the blockage of blood-brain barrier and peripheral side-effects. OBJECTIVE: To explore the feasibility of long term intranasal delivery of bone marrow stromal cells for treatment of brain injury caused by ischemia reperfusion. METHODS: Bone marrow stromal cells were sorted from rat whole bone marrow by adherent culture. Brain ischemia reperfusion rats were randomly divided into experimental group or control group. Bone marrow stromal cells or phosphate buffer saline were dropped into the nasal cavity of experimental group and control group respectively, once every 2 days for a total of 4 weeks. Rat behaviors were assessed once a week. Brain pathological examination was performed after the last behavior assessment. RESULTS AND CONCLUSION: Compared with the control group, the results of modified neurological severity score and Morris water maze test showed that rat behaviors of experimental group were gradually improved at 2 weeks after treatment (P < 0.05, P < 0.01). It corresponded to behavior improvement, and ischemia reperfusion resulted in 66% cell loss in hippocampal CA1 region in the control group; however, in the experimental group, the cell loss in hippocampal CA1 region was significantly lower to 25% (P < 0.01). Our data indicated that long term intranasal delivery of bone marrow stromal cells can improve the brain injury caused by ischemia reperfusion.关键词
脑损伤/骨髓基质细胞/鼻腔/血脑屏障/缺血再灌注/干细胞分类
医药卫生引用本文复制引用
王洋,沈慧娟,朱群娥,王飞,许卫珍,俞千,曾宪智..经鼻腔长期输送骨髓基质细胞改善大鼠缺血再灌注性脑损伤[J].中国组织工程研究,2012,16(27):5072-5075,4.基金项目
浙江省大学生科技创新项目(2010R417021) （2010R417021）
浙江省自然科学基金项目(Y2110158). （Y2110158）
