中国组织工程研究2012，Vol.16Issue(27)：5076-5080,5.DOI:10.3969/j.issn.2095-4344.2012.27.026
乌司他丁联合脐带间充质干细胞移植脊髓损伤大鼠后肢运动功能和运动诱发电位的变化
Effects of ulinastatin application combined with umbilical cord mesenchymal stem cells transplantation on changes in motor evoked potential and motor function of hindlimbs in rats with spinal cord injury
张占修 1李志远 1申勇2
作者信息
- 1. 邢台市人民医院关节骨科,河北省邢台市,050051
- 2. 河北医科大学第三医院脊柱骨科,河北省邢台市,050051
- 折叠
摘要
Abstract
BACKGROUND: Ulinastatin can reduce the inflammatory response, and get rid of oxygen free radicals. Ulinastatin has a protective effect on the central nervous system injury, and can effectively increase the survival rate of transplanted cells after spinal cord injury.
OBJECTIVE: To investigate the effects of umbilical cord mesenchymal stem cells transplantation and ulinastatin on the repair of motor function of hindlimbs in rats with spinal cord injury.
METHODS: Wistar rats were used to establish the spinal cord injury models and the models were randomly divided into four groups: control group, umbilical cord mesenchymal stem cells group, ulinastatin group and combination group. The models in the control group underwent tail vein injection of culture medium and intraperitoneal injection of normal saline. The umbilical cord mesenchymal stem cells group was injected with umbilical cord-matrix stem cells via the tail vein. The ulinastatin group underwent intraperitoneal injection of ulinastatin and the combination group underwent tail vein injection of umbilical cord mesenchymal stem cells and intraperitoneal injection of ulinastatin.
RESULTS AND CONCLUSION: At 4 weeks after transplantation, the function of rat hindlimbs in the combination group was superior to that in the umbilical cord mesenchymal stem cells transplantation group and the ulinastatin group (P < 0.05), and the hindlimb function was superior in the umbilical cord mesenchymal stem cells transplantation group and the ulinastatin group to that in the control group (P < 0.05). The number of PKH26-positive cells in the combination group was greater than that in the umbilical cord mesenchymal stem cells transplantation group, and the number was also significantly greater in the umbilical cord mesenchymal stem cells transplantation group than that in the ulinastatin group and control group (P< 0.01). At 8 weeks after transplantation, the latency and amplitude of somatosensory evoked potential and motor evoked potential in the combination group were significantly better than those in the other three groups (P < 0.05, P < 0.01). Umbilical cord mesenchymal stem cells transplantation combined with the ulinastatin can promote the regeneration of nerve synapses in rats with spinal cord injury, and can improve their motor function and electrophysiological function. The umbilical cord mesenchymal stem cells transplantation combined with the ulinastatin produces better effects than single use in repair of motor function of hundlimbs of rats with spinal cord injury.关键词
乌司他丁/脊髓损伤/脐带间充质干细胞/运动功能/细胞移植/干细胞分类
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张占修,李志远,申勇..乌司他丁联合脐带间充质干细胞移植脊髓损伤大鼠后肢运动功能和运动诱发电位的变化[J].中国组织工程研究,2012,16(27):5076-5080,5.
