中国组织工程研究2012，Vol.16Issue(28)：5141-5144,4.DOI:10.3969/j.issn.2095-4344.2012.28.003
葛根素抑制1,25-二羟基维生素D3促破骨细胞骨吸收功能的最佳剂量
Inhibitory effect of puerarin on osteoclastic bone resorption induced by 1,25-dihydroxyvitamin D3 in vitro: The optimal concentration of puerarin
摘要
Abstract
BACKGROUND: Many studies have focused on the effect of puerarin on the bone formation. However, the inhibitory effect of puerarin on osteoclastic bone resorption induced by 1,25-dihydroxyvitamin D3 in vitro remain poorly understood. OBJECTIVE: To study the inhibitory effect of different concentrations of puerarin on osteoclastic bone resorption induced by 1,25-dihydroxyvitamin D3 in vitro. METHODS: Bone marrow cells were isolated from 3-week-old mice and added to 24-well plates. The cells in the experiment groups were cultured in 1,25-dihydroxyvitamin D3+10%+alpha-modified minimum Eagles mediurn+various concentrations of puerarin (10, 50 and 100 ug/L). The control group was not treated with puerarin. RESULTS AND CONCLUSION: Osteoclast-like cells were formed and were not significantly different among the four groups. As to the concentration of alkaline phosphatase in the supernatant liquid, there were no significant differences among the four groups (P > 0.05), but the concentration of alkaline phosphatase in the 100 ug/L puerarin group was significantly higher than that of 0 and 50 ug/L puerarin on day 3 (both P < 0.01). The concentration of Ca2+ in the 10 ug/L puerarin group was significantly lower than that in the control group on days 3, 5 and 12 (P < 0.05-0.01), the same as the 50 ug/L puerarin group on day 12 (P< 0.01). Compared with the 10 ug/L puerarin group, the concentration of Ca2+ in the 100 ug/L puerarin group was significantly higher for up to day 12 (P < 0.05). The results demonstrat that the best dose of puerarin which has the inhibitory effect on osteoclastic bone resorption induced by 1,25-dihydroxyvitamin D3 in vitro is between 10-50 ug/L.关键词
葛根素/1,25-二羟基维生素D3/破骨细胞/骨吸收/抑制/组织构建分类
医药卫生引用本文复制引用
刘浩,李斌斌..葛根素抑制1,25-二羟基维生素D3促破骨细胞骨吸收功能的最佳剂量[J].中国组织工程研究,2012,16(28):5141-5144,4.基金项目
国家自然科学基金项目(30901671). （30901671）
