中国组织工程研究2012，Vol.16Issue(28)：5232-5236,5.DOI:10.3969/j.issn.2095-4344.2012.28.021
氟对成纤维细胞核心结合因子α1及增殖活性量-效关系的影响
Effect of fluoride on the expression of core binding factor alpha 1 in fibroblasts and dose-effect relationship between fluoride and proliferation activity of fibroblasts
摘要
Abstract
BACKGROUND: Fluoride may play an important role in extraperiosteal ossification of severe disabled skeletal fluorosis through inducing osteogenic differentiation of fibroblasts. Core binding factor alpha 1 (Cbfal), as a specific transcription factor of osteoblasts, is a necessary condition for osteoblast differentiation and osteogenesis. OBJECTIVE: To study the changes in Cbfal expression and proliferation activity of fibroblasts when skin fibroblasts from infant rats cultured in vitro were exposed to fluoride at different concentrations and different time. METHODS: Supernatant of control group and seven fluoride exposed groups (0.000 1, 0.001, 0.01, 0.1, 1,10 and 20 mg/L) were collected at 24, 48, 72 and 96 hours after fluoride, and the Cbfal was determined by enzyme linked immunosorbent assay. In addition, for studying the effect of fluoride on proliferation activity of fibroblast, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method was employed. RESULTS AND CONCLUSION: The Cbfal of fibroblast was quantitatively increased by fluoride, and played an important part in the developing of extraperiosteal ossification of skeletal fluorosis. The effect of fluoride on proliferation activity of fibroblast presented in a dose-time-effect dependent manner. Shorter time and lower dose of fluoride could enhance this activity; however, as the dose increasing and time expanding, this activity was significantly decreased.关键词
氟/成纤维细胞/核心结合因子α1/增殖活性/原代培养分类
医药卫生引用本文复制引用
刘晓利,李长城,刘克俭,崔彩岩,张裕曾,刘芸,黄安斌,余达林..氟对成纤维细胞核心结合因子α1及增殖活性量-效关系的影响[J].中国组织工程研究,2012,16(28):5232-5236,5.基金项目
国家自然科学基金资助项目(81072255). （81072255）
