中国组织工程研究2012，Vol.16Issue(28)：5287-5290,4.DOI:10.3969/j.issn.2095-4344.2012.28.032
钙网蛋白在心肌组织细胞氧化应激与缺血损伤构建中的表达与应用
Expression and application of calreticulin in the construction of cardiac histiocyte oxidative stress and ischemic injury
摘要
Abstract
BACKGROUND: Calreticulin regulates many physiological and pathophysiological processes in vivo, such as cell apoptosis, stress and cardiovascular inflammatory response. OBJECTIVE: To analyze the expression and application of calreticulin in ischemia-reperfusion injury. METHODS: An online search of PubMed and CNKI databases was performed for relevant articles published from 1996 to 2010. Articles on the structure and function of calreticulin, the role of calreticulin in autoimmunization and cardiac development, the expression change of calreticulin in the ischemia-reperfusion process were included. On that basis, the expression changes of calreticulin in oxidative stress and ischemia-reperfusion injury were observed by experiment. RESULTS AND CONCLUSION: Calreticulin plays an important role in the genesis, development and pathological changes of hearts by assisting protein folding correctly, maintaining the intracellular Ca2+ homeostasis as well as involving in the regulation of apoptosis, adhesion and the expression of steroid sensitive genes. Calreticulin expression in myocardial ischemia-reperfusion injury was detected by enzyme-linked immunosorbent assay, immunohistochemistry and RT-PCR using myocardial ischemia-reperfusion injury model in rats. The results indicate that calreticulin expression is increased after myocardial ischemia-reperfusion injury, and then the apoptosis signal pathway in endoplasmic reticulum is activated, leading to cell injury and aggravate the myocardial injury.关键词
钙网蛋白/心肌缺血-再灌注损伤/检测方法/蛋白表达/心脏分类
医药卫生引用本文复制引用
李雷勇,田岳凤,袁叶,王军,张斌仁,王旭..钙网蛋白在心肌组织细胞氧化应激与缺血损伤构建中的表达与应用[J].中国组织工程研究,2012,16(28):5287-5290,4.基金项目
国家自然科学基金资助项目(30873240) （30873240）
山西省自然基金项目(2010011056-1). （2010011056-1）
