中国组织工程研究2012，Vol.16Issue(29)：5336-5340,5.DOI:10.3969/j.issn.2095-4344.2012.29.004
直径可控阳极氧化铝表面有序纳米孔阵列对脐静脉内皮细胞黏附行为的影响
Ordered nanopore arrays on the surface of diameter-controlled anodic alumina influence the adhesion behavior of umbilical vein endothelial cells
摘要
Abstract
BACKGROUND: Studies have shown that many types of cells can influence the surface structure of micrometer scale on material surfaces. OBJECTIVE: To observe the effect of various pore sizes of ordered nanopore arrays on diameter-controlled anodic alumina on the adhesion behavior of human umbilical vein endothelial cells (hUVECs). METHODS: The order arranged porous anodic alumina (PAA) with controllable pore sizes (25, 50, 65 or 75 nm) was prepared using a two-step anodizing procedure in oxalic acid electrolytes. The hUVECs were seeded on the PAA surface with different pore sizes to evaluate the cellular adhesion behavior on the PAA surface. RESULTS AND CONCLUSION: The hUVECs adhered well on the surfaces of all the PAA specimens and showed a normal morphology. MTT assay showed that the hUVECs adhesion rates were significantly higher on the surface of the PAA with larger diameter of 75 nm compared with the PAA with smaller diameter of 25 nm after 4 and 24 hours of culture (P< 0.05). It is indicated that the PAA with larger diameter of 75 nm can promote the cell adhesion compared with the PAA with smaller diameter of 25 nm based on its better biological activity.关键词
阳极氧化铝/表面形貌/纳米多孔/人脐静脉内皮细胞/黏附分类
医药卫生引用本文复制引用
倪似愚,张燕飞,倪世容..直径可控阳极氧化铝表面有序纳米孔阵列对脐静脉内皮细胞黏附行为的影响[J].中国组织工程研究,2012,16(29):5336-5340,5.基金项目
国家自然科学基金项目(50902020) （50902020）
中央高校基本科研业务费专项资金资助项目(2011D10524). （2011D10524）
