中国组织工程研究2012，Vol.16Issue(29)：5375-5378,4.DOI:10.3969/j.issn.2095-4344.2012.29.012
多孔型丝素蛋白/羟基磷灰石复合骨髓间充质干细胞修复兔半月板无血运区软骨损伤
Repair of meniscal cartilage injuries in the avascular zone of rabbits by using porous silk fibroin/hydroxyapatite combined with bone marrow mesenchymal stem cells
朱现玮 1徐卫袁 2张兴祥 2王创利 2王黎明 2沙卫平 2朱寅1
作者信息
- 1. 江苏大学临床医学院,江苏省镇江市,212013
- 2. 张家港市第一人民医院骨科,江苏省张家港市,215600
- 折叠
摘要
Abstract
BACKGROUND: With the emergence of tissue engineering, the possibility of meniscal repair in the avascular zone is greatly increased, but single scaffold materials fail to meet an ideal scaffold and have certain limitations.
OBJECTIVE: To investigate the effect of silk fibroin/hydroxyapatite (SF/HA) combined with bone marrow mesenchymal stem cells (BMSCs) on repairing meniscal cartilage injuries in the avascular zone of rabbits.
METHODS: Rabbit BMSCs were isolated, cultured and induced into fibrochondrocytes, and then cultured with SF/HA. A rabbit model of unilateral meniscal cartilage injuries in the avascular zone was established. A total of 42 rabbits were randomly divided into three groups. Compound group was implanted with the compound of BMSCs and SF/HA, pure material group was implanted with SF/HA and control group had no treatment.
RESULTS AND CONCLUSION: The results of general observation showed that the injured tissue had been filled in the compound group. And the repair effect was improved gradually at weeks 8-12, which was similar to the normal meniscus tissue and superior to the other two groups. The results of histological examination exhibited cartilage capsule and collagen fibers arranged in disorder at week 8, and meniscus injuries were completely repaired in the compound group at week 12. Meniscus injuries were partly repaired with scar healing in the pure materials group. The control group was not found cartilage injuries healing. The results of MRI examination indicated that the repair effect of the compound group was the best. These findings suggest that the method of BMSCs combined with SF/HA is feasible to repair meniseal cartilage injuries in the avascular zone, and the SF/HA can be used as scaffold materials for meniscal cartilage tissue engineering due to its good biocompatibility.
关键词
丝素蛋白/羟基磷灰石材料/骨髓间充质干细胞/软骨损伤/半月板/生物材料分类
医药卫生引用本文复制引用
朱现玮,徐卫袁,张兴祥,王创利,王黎明,沙卫平,朱寅..多孔型丝素蛋白/羟基磷灰石复合骨髓间充质干细胞修复兔半月板无血运区软骨损伤[J].中国组织工程研究,2012,16(29):5375-5378,4.
