中国组织工程研究2012，Vol.16Issue(29)：5412-5416,5.DOI:10.3969/j.issn.2095-4344.2012.29.019
小口径生物型人工血管移植后的血管内皮再生
Vascular endothelial regeneration after small-diameter bioartificial vascular graft
林绿标 1许益民 1漆松涛 2曾绍文 3李伟秋 4庄冰容4
作者信息
- 1. 汕头大学医学院第一附属医院神经外科,广东省汕头市,515041
- 2. 南方医科大学南方医院神经外科,广东省广州市,510515
- 3. 汕头市中心医院病理科,广东省汕头市,515041
- 4. 汕头大学医学院中心实验室,广东省汕头市,515000
- 折叠
摘要
Abstract
BACKGROUND: Characteristics of vascular prostheses are similar to the natural vessels and it has high histocompatibility after transplantation. But endothelial regeneration is the key for vascular prosthesis unblocked eternally.
OBJECTIVE: To investigate the histocompatibility of a novel small-diameter bioartificial vascular graft at different periods postoperatively and the histopathologic changes following intimal regeneration of the vessel wall.
METHODS: A dog model of artificial vascular grafts implanted end-to-end by a continuous suture in the common carotid artery was established.
ESULTS AND CONCLUSION: ㎜ight microscope results showed that at week 12 after graft, endothelial cells were sparsely distributed throughout the neointima surface near the stoma; at month 6 after graft, endothelial cells were presented on the whole surface of the patent graft lumen; 1.5 years after graft, lumens of graft vessels were unobstructed, and parts of endomembrane tissues were found chronic inflammation. 〦lectron microscope results showed that at week 12 after graft, new vascular endothelial cells were in fusiform shape and arranged regularly; besides, the endothelial cells spread from the stoma to the middle of the graft vessel. After graft for 6 months, the endothelial cells spread from the stoma to the middle of the graft vessel. Endothelial cell colony in the middle of the graft vessel showed leaping and flake-like growth, and the cells were arranged more compactly; moreover, their shapes were more like the endothelial cells of mature vessels. After graft for 1.5 years, the whole vessel wall was covered by tightly arranged endothelial cells, and parts of endomembrane tissues revealed chronic inflammation. These findings suggest that the novel small-diameter bioartificial vascular graft has many advantages, for example, the newborn intima forms early and completely, and endangium can be well reconstructed; in addition, it has good biocompatibility and stability.
关键词
小口径/生物型人造血管/组织病理学/血管内皮细胞/颈总动脉/犬分类
医药卫生引用本文复制引用
林绿标,许益民,漆松涛,曾绍文,李伟秋,庄冰容..小口径生物型人工血管移植后的血管内皮再生[J].中国组织工程研究,2012,16(29):5412-5416,5.
