中国组织工程研究2012，Vol.16Issue(29)：5433-5436,4.DOI:10.3969/j.issn.2095-4344.2012.29.023
可塑型脱矿骨基质/海藻酸钠复合材料的制备及细胞相容性
Preparation and cell compatibility of demineralized bone matrix/sodium alginate composite
摘要
Abstract
BACKGROUND: It is important to prepare a bone substitute material with good osteoacusis, osteoinductive activity and cell compatibility that is easily molded, which is of significance to repair irregular bone defects and refractory bone fractures. OBJECTIVE: To prepare demineralized bone matrix/sodium alginate (DBM/ALG) composite material and to screen out the optimal composite ratio, as well as to evaluate its cell compatibility. METHODS: DBM was prepared from the cortical bone of healthy donors by cleaning, grinding and demineralization. According to the ratio of 5/5, 6/4, 7/3, 8/2, the DBM was compounded with ALG, and the DBM/ALG composite material was prepared through screening the optimal ratio by disperse diameter method. L-929 fibroblasts of mice were cultured in the leaching liquor of DBM/ALG, and the cytotoxicity was tested by MTT method at days 2, 4 and 7. RESULTS AND CONCLUSION: With the increase of ALG content, the viscosity of DBM/ALG was strengthened and dispersed diameter was reduced gradually. When the ratio of DBM/ALG was 7/3, DBM/ALG was not discrete easily, and dispersed diameter was 3.7 cm in 37 癈 water. The results of MTT showed that cell proliferation rates were 91.56%, 95.43% and 97.47% at days 2, 4 and 7, respectively; while cytotoxicity was in grade 1.关键词
脱钙骨基质/海藻酸钠/离散度/细胞相容性/骨修复材料/生物材料分类
医药卫生引用本文复制引用
徐伟俊,李宝兴,张育敏,刘晓明,李靖,王旭昇 ,马绍英..可塑型脱矿骨基质/海藻酸钠复合材料的制备及细胞相容性[J].中国组织工程研究,2012,16(29):5433-5436,4.基金项目
山西省青年科学基金项目(2006021050). （2006021050）
