中国组织工程研究2012，Vol.16Issue(31)：5711-5714,4.DOI:10.3969/j.issn.2095-4344.2012.31.003
猪劈离式原位肝移植动物模型建立及麻醉处理
Establishment of a porcine splitting orthotopic liver transplantation model and management of anesthesia
孙建军 1陈国勇 1汤高枫 1陈建斌 1王谦 1谢占涛 1赵会博 1季节1
作者信息
- 1. 郑州人民医院,河南省郑州市,450003
- 折叠
摘要
Abstract
BACKGROUND: The porcine splitting orthotopic liver transplantation technique is characterized as high incidence of biliary complication and ischemic necrosis of the left medial lobe. In order to further research the splitting liver transplantation, a suitable animal is need to establish the transplantation and anesthesia model.
OBJECTIVE: To investigate the establishment of porcine splitting orthotopic liver transplantation model and the characteristics of anesthesia management.
METHODS: Forty 3-month-old pigs weighing 25-30 kg were selected, the veno-venous bypass was performed under general anesthesia, and then the donor liver was split along Taira line. After the section was trimmed, the orthotopic liver transplantation was performed.
RESULTS AND CONCLUSION: The liver function recovered well, and the survival rate after one week was 50%, the operative time of recipient was (220?1) minutes, the recipient underwent anhepatic period was (35?) minutes. Ten animals survived more than 48 hours. During anhepatic and reperfusion phase, the hemodynamics was significantly changed; and in the reperfusion phase, the content of serum potassium was significantly increased while the temperature was decreased. The result indicates that: 甌he porcine splitting orthotopic liver transplantation model (along Taira line) is easy to operate, and has highly standardized degree and success rate as well as good reproducibility and stability, which is considered as an ideal animal model for a series of studies of liver transplantation in large animals. 〦ven in a good vein bypass, the changes of hemodynamics and environment in anhepatic and reperfusion phase were significant. The correction of clotting mechanism and maintaining a stable internal environment during anesthesia are critical for the success of the operation.关键词
劈离式/原位肝移植/肝脏移植/猪/模型/Taira线/麻醉/器官移植分类
医药卫生引用本文复制引用
孙建军,陈国勇,汤高枫,陈建斌,王谦,谢占涛,赵会博,季节..猪劈离式原位肝移植动物模型建立及麻醉处理[J].中国组织工程研究,2012,16(31):5711-5714,4.
