中国组织工程研究2012，Vol.16Issue(31)：5783-5787,5.DOI:10.3969/j.issn.2095-4344.2012.31.019
无心跳供体肺移植中部分液体通气对肺保护的病理学评估
Pathological assessment of part liquid ventilation in the protection of non-heart-beating donor lung
摘要
Abstract
BACKGROUND: How to protect the non-heart-beating donor lung in the warm ischemia period in order to reduce the primary graft dusfunction after transplantation is the most important issue to all the researchers and clinicians. OBJECTIVE: To assess the effect of part liquid ventilation in the protection of non-heart-beating donor lung from pathology. METHODS: Thirty-six health SD rats were randomly divided into three groups. Homemade 16G deep vein needle lien was used for endotracheal intubation and connected to a respirator machine with mechanical ventilation. The KACL solution was injected into the jugular and lead to the rat sudden death, the multi-channel physiological detector was used to continuous record the blood pressure, the model was successful when the blood pressure was 0 mmHg. After the models were established, the models in the normal mechanical ventilation group were preformed with mechanical ventilation for 2 hours; the models in the saline group and the perfluoro carbon group were given oxygen ventilation for 5 minutes, then injected with the equivalent of the functional residual capacity of the oxygen salt and high oxygen perfluoro carbons (10 mL/kg) by endotracheal intubation, and mechanical ventilation for 2 hours. RESULTS AND CONCLUSION: General observation showed that the lung of saline group was swelling, the sheet surface of lung tissue was bleeding, the lung was dark red, airway edema with bloody fluid; the lung of oxygen group was less swelling and less bleeding points of lung surface; in the perfluoro carbon group, the lung tissue inflammatory cell infiltration and edema was reduced. Light microscope observation of saline group and oxygen group showed that there was diffuse alveolar and interstitial hyperemia and edema, a large number of inflammatory cell infiltration could be seen in the bronchial wall and capillaries surrounding, the alveolar was over-expansion and the alveolar wall was ruptured; the lung tissue structure in the perfluoro carbon group was more complete and no significant damage. The results show that partial liquid ventilation can play a very good role in the protection of the non-heart-beating donor lungs.关键词
部分液体通气/无心跳供体肺移植/病理学/热缺血/氟化碳分类
医药卫生引用本文复制引用
闫宇博,张翔宇,李吉尧,崔键,张凯,曹守强,刘成,辛衍忠,赵桂彬,韩敬泉,董庆..无心跳供体肺移植中部分液体通气对肺保护的病理学评估[J].中国组织工程研究,2012,16(31):5783-5787,5.基金项目
"哈尔滨市优秀学科带头人"基金资助(2011RFXYS077),项目名称:无心跳供体肺移植中肺泡Ⅱ型上皮细胞凋亡的研究. （2011RFXYS077）
