中国组织工程研究2012，Vol.16Issue(31)：5788-5792,5.DOI:10.3969/j.issn.2095-4344.2012.31.020
桂附地黄丸干预主要组织相容性复合物半相合移植小鼠慢性移植物抗宿主病的效应
Effect of Guifu rehmaniae bolus on preventing chronic graft versus host disease in major histocompatibilty complex halpo-identical bone marrow transplantation mice
摘要
Abstract
BACKGROUND: At present, the method for the treatment of chronic graft versus host disease is not ideal due the adverse reactions, founding the traditional Chinese medicine for effective intervention and treatment of chronic graft versus host disease is one of the common focuses of attention for the transplant scholars. OBJECTIVE: To observe effect of Guifu rehmaniae bolus on preventing chronic graft versus host disease in major histocompatibilty complex halpo-identical bone marrow transplantation mice. METHODS: Transplantation models of major histocompatibilty complex halpo-identical hematopoietic stem cells transplantation mice were established by transplanting the hematopoietic stem cells of male Balb/cH-2d mice to female (Balb/cxC57BL/6) F1 H-2d/b (CB6F1) mice. After transplantation, 24 recipient mice were divided into two groups: Guifu rehmaniae bolus group and blank group, mice in the Guifu rehmaniae bolus group were lavaged from the first day after transplantation with 0.2 mL Guifu rehmaniae bolus liquid twice per day; mice in the blank group were lavaged with the same dose of normal saline and lasted for 100 days. RESULTS AND CONCLUSION: Five mice died in the Guifu rehmaniae bolus group, and all mice died in the blank group at 86 days after transplantation (P=0.004). The mice in the blank group depressed at 35 days after transplantation and the mice in the Guifu rehmaniae bolus group depressed at 45 days after transplantation, and the clinical score in the blank group was significantly higher than that in the Guifu rehmaniae bolus group (P < 0.05). Pathological observation showed that the mice with chronic graft versus host disease in Guifu rehmaniae bolus group mostly classified from 0 to 1 grade. Compared with blank group, the liver, skin and small intestine tissue of the mice in Guifu rehmaniae bolus group were improved. Guifu rehmaniae bolus can relieve symptoms of chronic graft versus host disease in major histocompatibilty complex halpo-identical bone marrow transplantation mice effectively and prolong their survival time, and improve histologic manifestations.关键词
主要组织相容性复合物/半相合移植/慢性移植物抗宿主病/桂附地黄丸/造血干细胞移植分类
医药卫生引用本文复制引用
吴顺杰,梁凯雯,吴远彬,周健,涂三芳..桂附地黄丸干预主要组织相容性复合物半相合移植小鼠慢性移植物抗宿主病的效应[J].中国组织工程研究,2012,16(31):5788-5792,5.基金项目
广州中医药大学科研创新基金资助项目(2007C064). （2007C064）
