中国组织工程研究2012，Vol.16Issue(31)：5837-5842,6.DOI:10.3969/j.issn.2095-4344.2012.31.029
纤维连接蛋白在肾间质纤维化大鼠肾组织中的表达
Expression of fibronectin in the kidney of rats with renal interstitial fibrosis
程红新 1杨晓萍 1蒋雅红 2林智峰 1赵瑾 3陶林3
作者信息
- 1. 石河子大学医学院第一附属医院,肾病科,新疆维吾尔自治区石河子市,832008
- 2. 石河子大学医学院第三附属医院医务部,新疆维吾尔自治区石河子市,832000
- 3. 石河子大学医学院第一附属医院,病理科,新疆维吾尔自治区石河子市,832008
- 折叠
摘要
Abstract
BACKGROUND: The increasing of the fibronectin expression plays an important role in the process of renal interstitial fibrosis.
OBJECTIVE: To investigate the expression of fibronectin in the kidney of the rats with renal interstitial fibrosis.
METHODS: Seventy-two male Sprague-Dawley rats were randomly divided into normal control group (r>=24), sham-operation group (n=24) and model group (n=24) by touching ball method. Rats in the model group were used to establish the unilateral ureteral obstruction model, the rats in the sham-operation group received the open surgery and the left ureter was freed, and the rats in the normal control group received no treatment.
RESULTS AND CONCLUSION: Histological observations of renal tissues revealed that the degree of renal tubulointerstitial fibrosis of rats in the model group was significantly increased when compared with the normal control group and sham-operated group after unilateral ureteral obstruction, and it was increased gradually accompanying with the increasing of obstruction degree. The expression of fibronectin, matrix metalloproteinase-9 and transforming growth factor pi in renal tissue of model group was significantly increased, and it was increased gradually accompanying with the increasing of obstruction degree, they all reached the maximum at the 14th day after modeling, there was significant difference in the model group when compared with normal control group and sham-operation group (P < 0.05). It indicated that in the process of the renal interstitial fibrosis in unilateral ureteral obstruction rats, the expression of fibronectin was elevated from the early stage of renal injury and positively associated with fibrosis stage, and then promoted the renal fibrosis. The higher protein expression of matrix metalloproteinase-9 might be one of reasons for increasing the renal interstitial fibrosis.关键词
单侧输尿管梗阻模型/纤维连接蛋白/基质金属蛋白酶9/转化生长因子β1/肾间质纤维化分类
医药卫生引用本文复制引用
程红新,杨晓萍,蒋雅红,林智峰,赵瑾,陶林..纤维连接蛋白在肾间质纤维化大鼠肾组织中的表达[J].中国组织工程研究,2012,16(31):5837-5842,6.
