新疆农业科学2012，Vol.49Issue(5)：862-867,6.DOI:10.6048/j.issn.1001-4330.2012.05.014
东方百合OT系列试管鳞茎的诱导与植株再生体系的建立
The Inducing of OT Oriental Lily Bulblet and Establishing of Regeneration System
摘要
Abstract
[Objective ] The paper studied establishing regeneration system by inducing bulblet of OT oriental lily varieties of Concad and Robina. It is basis for the further development of virus - free fast propagation system. [ Method] The induction of difficulty of die degree and speed with different parts of the explants were compared, and optimum level of induction hormones, propagation hormones and proliferation rooting hormone were selected so as to establish propagation system of Concad and Robina. [ Result ] The result showed that the best material was bulblet tip with bulblet of OT oriental lily Concad and Robina. Because Concad and Robina themselves contain different endogenous hormones, BA of the best concentration in proliferation medium was controlled within 1. 0 -0. 5 mg/L. For rooting hormones, Robina was NAA0. 5 mg/L, Concad's NAA0. 3 mg/L, the rooting rate were all 100%. Tube seedlings transplant was conducted better with the hill soil, and the survival rate could reach as high as 93% . [ Conclusion ] The best material was bulblet tip with bulblet of OT oriental lily Concad and Robina. The Robinas proliferation culture medium was MS + BAO. 5 mg/L + NAAO. 5 mg/L, Concad's was MS + BAO. 5 mg/L + NAAO. 1 mg/L. Robina's rooting hormone was NAAO. 5 mg/L, Concads was NAAO. 3 mg/L. The best soil was mountain soil for transplanting plantlets.关键词
东方百合OT系/试管鳞茎/激素/再生快繁体系Key words
OT oriental lily/ bulblet/ hormone/ regeneration micropropagation system分类
农业科技引用本文复制引用
廖晴,周斌,玛尔哈巴,刘巧玲,盛玮..东方百合OT系列试管鳞茎的诱导与植株再生体系的建立[J].新疆农业科学,2012,49(5):862-867,6.基金项目
新疆维吾尔自治区科技成果转化项目(201154111) （201154111）
