新疆农业科学2012，Vol.49Issue(5)：940-944,5.DOI:10.6048/j.issn.1001-4330.2012.05.026
细毛羊羔羊超数排卵及体外胚胎生产移植
Fine Wool Sheep Superovulation and in vitro Lamb Embryos Production Transplantation
摘要
Abstract
[Objective]The experiment was conducted to study the in vitro fertilization effect of lamb age, lamb weight, superovulation method sperm - oocyte co - incubation time on fine wool lamb superovulation and oocyte. [ Method] Domestic 4-9 weeks FSH lambs were chosen to go through two superovulation methods processing. 1 032 oocytes available were recovered, and after in vitro fertilization and transplantation receptors were 98, lambing 25. [ Result]The number of retrieved oocytes and oocyte number available of different weeks old lamb are greatly different (P ＜0.05); the number of different hormone treatment group growth follicle two treatment options in the development showed no significant difference (P ＞0.05) , but the recovery rate and the number of oocytes available were significantly different (P ＜ 0.05 ) ; there was a big difference (P ＜ 0.05 ) between the growth follicle number and oocyte number available. [ Conclusion]The results showed that 4 to 6 weeks of age of fine - wool sheep lamb superovulated oocytes obtained quite a lot of numbers, the number of repeated superovulation and the sheep superovulation effect is not ideal; at the same time, the semen pH value affected was adjusted to be between 7. 5 and 7. 6, and the sperm - oocyte co - incubation time control was within 22 -26 h. Oocytes in vitro fertilization and development effect was good.关键词
幼羔/超排/卵母细胞/胚胎Key words
lamb/ superovulation/ oocytes/ embryo分类
农业科技引用本文复制引用
陈童,郝耿,杨会国,马桢,郭志勤..细毛羊羔羊超数排卵及体外胚胎生产移植[J].新疆农业科学,2012,49(5):940-944,5.基金项目
国家肉羊产业技术体系阿勒泰综合试验站 （）
