畜牧兽医学报2012，Vol.43Issue(4)：509-515,7.
FSHR基因第1外显子多态性及其与小梅山猪产仔数的关系
Polymorphism of Exon1 of FSHR Gene and Its Relationship with Litter Size in Xiaomeishan Pigs
摘要
Abstract
The present study was to detect the polymorphism of exonl of follicle-stimulating hormone receptor (FSHR) gene and investigate the relationship between FSHR gene and litter size in pigs. The polymorphism of exonl of FSHR gene was investigated by PCR-SSCP and direct sequencing methods and the effect of exonl of FSHR gene on litter size in Xiaomeishan pigs was analyzed using the least square analysis, simultaneously the variance components were analyzed and the selective reaction was predicted. The results showed that three SNPs (C70T, C74G and C81T) were detected in exonl of FSHR gene, of which C74G caused amino acid change. Simultaneously three haplotypes and five genotypes were screened out. The difference of FSHR gene haplotypes and genotypes distribution among Xiaomeishan pigs, Fengjing pigs and Large White pigs reached extremely significant level (P<0. 01). According to the result of correlation analysis, in Xiaomeishan pigs, the TNB of the sows with genotype A A and AC after the second parity were 0. 64 (P>0. 05) and 0. 36 (P>0. 05) piglets less than those with genotype BC, and the TNB and NBA of the sows with genotype AA in all parities were 0. 80 (P<0. 01) and 0. 67 (P< 0. 01) piglets less than those with genotype AC. The heredity of TNB and NBA in Xiaomeishan pigs was mostly influenced by the genetic dominant effect in exonl of FSHR gene.关键词
小梅山猪/FSHR/PCR-SSCP/产仔数Key words
Xiaomeishan pigs/ FSHR/ PCR-SSCP/ litter size分类
农业科技引用本文复制引用
吴井生,张跟喜,戴国俊,叶兰,王金玉..FSHR基因第1外显子多态性及其与小梅山猪产仔数的关系[J].畜牧兽医学报,2012,43(4):509-515,7.基金项目
江苏省农业三项工程项目(sx<2009>25,sx<2010>244) （sx<2009>25,sx<2010>244）
江苏省畜禽技术改进项目(2009<46>) （2009<46>）
