畜牧兽医学报2012，Vol.43Issue(4)：553-557,5.
生长因子对兔骨髓间充质干细胞增殖的影响
Proliferative Effects of Growth Factors on Bone Marrow Mesenchymal Stem Cells of Rabbits in vitro
摘要
Abstract
To study the effects of basic fibroblast growth factor (bFGF) on growth and proliferation of rabbit bone marrow mesenchymal stem cells (BMSCs) in vitro, rabbit BMSCs were cultured and identified in vivo, treated with bFGF at final concentration of 5, 10, 20, 40, 80, 100 μg · L-1. Cell growth and proliferation were detected by MTT assay and flow cytometer. MTT assay showed that proliferation promotion effect of bFGF nearly reached the peak at 80 μg · L-1 (P<0. 01). Compared with the negative control group, significant proliferation promotion effect was detected in the bFGF groups from 72 h(P<0. 01) ; Flow cytometer result showed that proliferation index of bFGF groups BMSCs was higher than that of control group(P<0. 01). Basic fibroblast growth factor can promote proliferation of rabbit BMSCs in vitro, 80 μg · L-1 is the optimum concentration for in vitro culture which can be used as the best culture condition of rabbit BMSCs proliferation in vitro.关键词
兔/骨髓间充质干细胞/碱性成纤维细胞生长因子(bFGF)/体外增殖Key words
rabbit/ bone marrow mesenchymal stem cells/ basic fibroblast growth factor/ proliferation in vitro分类
农业科技引用本文复制引用
和小娥,任卫青,邓立新,何俊丹,秦佳晨,吕婧玉,曹桂彬,王新庄..生长因子对兔骨髓间充质干细胞增殖的影响[J].畜牧兽医学报,2012,43(4):553-557,5.基金项目
河南省基础与前沿技术研究计划(092300410081) （092300410081）
现代农业产业技术体系专项资金(30300324) （30300324）
