畜牧兽医学报2012，Vol.43Issue(4)：580-587,8.
猪圆环病毒2型感染对猪瘟弱毒疫苗接种猪免疫应答的影响
Influence of Porcine Circovirus Type 2 Infection on Immune Response of Pigs to Classical Swine Fever Vaccine
摘要
Abstract
To study the influence of porcine circovirus type 2 (PCV2) infection on immune response of pig to classical swine fever (CSF) vaccine, twenty 28-day-old healthy weaning piglets were randomly divided into four groups of five (Group V-I, I-V, V and C). The piglets in Group V-I were intramuscularly injected with CSF vaccine two days before being inoculated with PCV2, piglets in Group I-V were infected by PCV2 two days before being injected with CSF vaccine, piglets in Group V were only vaccinated by CSF vaccine and Group C as the blank control without inoculating anything. Piglets were vaccinated twice at an interval of twenty-one days and blood samples were collected periodically so as to detect CSFV-specific serum antibody, lymphocyte proliferation activity and mRNA expression levels of IFN-γ, IL-2 and IL-4 in peripheral blood lymphocyte (PBLC). PCV2 infection, either before or after vaccination, reduced CSFV-specific serum antibody production, seroconversion and lymphocyte proliferation activity. After the primary vaccination, expression of IFN-y, IL-2, IL-4 and IL-10 mRNA decreased seriously in PBLC from piglets in Group V-I and Group I-V, especially in Group V-L Following the secondary vaccination, expressions of IFN-γ and IL-10 mRNA in PBLC from Group V-I and Group 1-V appeared seriously unbalanced and those of IL-2 as well as IL-4 mRNA were deficient, especially in Group I-V. In conclusion, PCV2 infection before or after vaccination with CSF vaccine affected porcine humoral and cellular immunity and led to expression suppression and unbalance of cyto-kines in PBLC.关键词
PCV2/猪瘟弱毒疫苗/抗体/淋巴细胞增殖活性/细胞因子Key words
PCV2/ classic swine fever vaccine/ antibody/ lymphocyte proliferation activityj cytokine分类
农业科技引用本文复制引用
赵丹萍,陶彬,陈立功,李艳琴,宋勤叶,李潭清,杨芳,李红园,袁洪兴..猪圆环病毒2型感染对猪瘟弱毒疫苗接种猪免疫应答的影响[J].畜牧兽医学报,2012,43(4):580-587,8.基金项目
河北省自然基金项目(C2010000673) （C2010000673）
国家自然基金项目(30771600) （30771600）
河北农业大学大学生科技创新活动基金 （）
