禽新型黄病毒RT-LAMP检测方法的建立OA北大核心CSCDCSTPCD
Establishment of Rapid Reverse Transcription Loop Mediated Isothermal Amplification for Detection of Avian Novel Flavivirus
根据环介导等温扩增技术(LAMP),建立了一种适用于禽新型黄病毒的逆转录环介导等温扩增(RT-LAMP)快速检测方法.根据禽新型黄病毒E蛋白基因序列,在保守区设计了一套针对禽新型黄病毒基因8个区域的6条特异性引物,并对反应条件进行优化.结果表明该方法对NDRV、GPV、MDRV、NDV、ILTV、FPV均无扩增反应,并可通过反应液是否有沉淀或向反应液中加入荧光染料来对结果进行可视化观察；扩增反应只需要在常规水浴锅中进行,45 min内可完成反应；…查看全部>>
A reverse transcription loop mediated isothermal amplification (RT-LAMP) assay was developed for detection of avian novel flavivirus virus. According to the sequences of avian novel flavivirus virus E protein gene, six primers specific to the eight sites of novel flavivirus virus gene were designed, and the reaction conditions were optimized. The results showed that the assay had no cross reaction with NDRV, GPV, MDRV, NDV, ILTV and FPV. The assay was perfor…查看全部>>
李兆龙;陈仕龙;林锋强;王劭;程晓霞;朱小丽;陈少莺
福建省农业科学院畜牧兽医研究所,福州350013福建省畜禽疫病防治工程技术研究中心,福州350013福建省农业科学院畜牧兽医研究所,福州350013福建省畜禽疫病防治工程技术研究中心,福州350013福建省农业科学院畜牧兽医研究所,福州350013福建省畜禽疫病防治工程技术研究中心,福州350013福建省农业科学院畜牧兽医研究所,福州350013
农业科技
禽新型黄病毒RT- LAMP检测方法
avian novel flavivirus loop-mediated isothermal amplification diagnosis method
《畜牧兽医学报》 2012 (4)
659-663,5
福建省农科院创新团队项目(STIF-Y02)福建省公益类科研院所专项(2011R1025-2)
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