畜牧兽医学报2012，Vol.43Issue(4)：664-668,5.
柔嫩艾美耳球虫Etron2基因在不同发育阶段转录水平差异的分析
Transcription Profiles of Etron2 Gene during the Different Development Stages
摘要
Abstract
The objective of this study was to study the transcription profiles of Etron2 gene during the different development stages. The parasites at different developmental stages were harvested and their total RNA were substracted by real-time PCR using Etactin gene as a reference for establishing standard curves to evaluate the copy number of Etronl genes. All of quantitative analyses were reliable because the melting curve showed a single peak. Etronl gene transcription reached a peak during the unsporulated oocysts stage, then merozoites, sporulated oocysts and the lowest during the sporozoites stage. It is likely that the transcription messages were stored during the unsporulated oocysts stage, and then the Etron2 protein can be translated and secreted into the rhoptry soon when the oocysts were sporulated, and it may play an important role in the process of invasion.关键词
柔嫩艾美耳球虫/棒状体颈部蛋白2/实时荧光定量PCR/转录水平Key words
Eimeria tenella/ Etron2 protein/ Real-time PCR/ transcription分类
农业科技引用本文复制引用
戚南山,孙铭飞,吴彩艳,廖申权,吕敏娜,袁建丰,余劲术,李祥瑞,蔡建平..柔嫩艾美耳球虫Etron2基因在不同发育阶段转录水平差异的分析[J].畜牧兽医学报,2012,43(4):664-668,5.基金项目
NSFC-广东联合基金项目(U0831004) （U0831004）
