畜牧兽医学报2012，Vol.43Issue(5)：701-707,7.
江淮水牛线粒体D-loop区和Cytb基因遗传多态性及系统发育分析
Genetic Variation and Phylogeney of Jianghuai Buffalo Based on Mitochondrial DNA D-loop and Cytb Gene
摘要
Abstract
To study the molecular germplasm characteristics of Jianghuai buffalo, the genetic diversity and phylogeny of mitochondrial DNA D-loop region and the complete sequence of cyto-chrome (Cyt b) gene of 82 Jianghuai buffaloes sampled from two subpopulations were investigated. These data were analyzed together with the published D-loop sequences of 141 Chinese buffaloes in GenBank. A total of 104 haplotypes and 91 nucleotide polymorphic sites were identified. Thirty-two D-loop haplotypes were newly found only in Jianghui buffalo. The estimated overall nucleotide diversity and haplotype diversity were (0.015 43 ± 0.001 42) and (0. 948±0. 009), respectively. The genetic variability of Jianghuai buffalo was similar with those of other Chinese buffalo populations, with nucleotide diversity to be (0. 014 89 ± 0. 002 32) and haplotype diversity to be (0. 955±0. 013), which indicated the rich mitochondrial genetic diversity in this population. Phylogenetic tree and network analysis revealed two mtDNA lineages, the A and B lines of swamp buffaloes, and the B line could be further divided into sub-lineages of bl and b2, which indicated that at least there were two maternal swamp buffalo origins in Jianghuai swamp buffalo population. The results based on the phylogenetic analysis of Cytb gene also supported this conclusion observaed in the D-loop region of Jianghuai buffalo. These findings lay a foundation for conservation and utilization of Jianghuai buffalo genetic resources in the future.关键词
水牛/mtDNA/D-loop/细胞色素b/遗传多样性/单倍型Key words
buffalo/ mtDNA/ D-loop/ cytochrome b/ genetic diversity/ haplotype分类
农业科技引用本文复制引用
卢永芳,蔡治华,李强,张毅,张勤,张沅..江淮水牛线粒体D-loop区和Cytb基因遗传多态性及系统发育分析[J].畜牧兽医学报,2012,43(5):701-707,7.基金项目
自然科学基金(30901015) （30901015）
国家科技支撑课题(2011BAD28B02) （2011BAD28B02）
