畜牧兽医学报2012，Vol.43Issue(5)：735-739,5.
体外培养猪小肠上皮细胞大豆凝集素结合位点的标记
In vitro Marker of Soybean Agglutinin Binding Sites in Porcine Intestinal Epithelial Cells
摘要
Abstract
This study aimed to culture in vitro and separate piglet small intestines epithelial cells and observe the soybean agglutinin binding sites. Newborn and health piglets with non-lactation were selected, and the intestinal epithelium cells were cultured and purified. The intestinal epithelium cells purified were identified by immunochemistry method taking the cell keratin 8 antibodies as the first antibody. The intestinal epithelium cells identified were used to mark the soybean agglutinin binding sites with FITC-SBA probe by cell agglutinin fluorescent chemistry marker test. The results showed that the porcine small intestinal epithelium cells purified were positive cells by cell keratin 8 antibodies identification, the purity was more than 90%, and the pig small intestine epithelial cells were positively marked by FITC-SBA. The porcine small intestinal epithelium cells cultured in vitro posses the soybean agglutinin binding sites, and further specify the porcine small intestinal epithelium cells is one of the main cells combined by soybean agglutinin, which provide the theory for the research on monogastric animal soybean agglutinin antinutrition mechanism.关键词
大豆凝集素/猪/小肠上皮细胞/荧光标记Key words
soybean agglutinin/ swine / intestinal epithelium cells/ fluorescent labeling分类
农业科技引用本文复制引用
车东升,穆成龙,刘飞飞,张海全,孙泽威,秦贵信..体外培养猪小肠上皮细胞大豆凝集素结合位点的标记[J].畜牧兽医学报,2012,43(5):735-739,5.基金项目
国家自然科学基金面上项目(30871802) （30871802）
吉林农业大学青年基金 （）
