畜牧兽医学报2012，Vol.43Issue(6)：915-921,7.
Clade2.3.2H5N1亚型禽流感疫苗候选株的构建
Generation of Clade 2.3.2 H5N1 Subtype Avian Influenza Vaccine Candidate
摘要
Abstract
Currently, H5N1 viruses in Clade 2. 3. 2 are predominant viruses which prevail in China and other countries, and occurrence of a certain evolutionary trends of amino acid changes. In this report, a reassortant virus containing the HA and NA genes from an new isolate A/chicken / Yangzhou/1117/2011(YZC3) and the backbone A/Puerto Rico/8/34 (HIND (PR8) strain were constructed. The virus was rescued from the COS-1 cell line and prepared as inactivated whole-virus vaccine. Vaccine virus was avirulent to SPF chickens and embryonated chicken eggs, replicating well in eggs and MDCK cells. The results indicate that the reassortant viruses is suitable for vaccine manufacturing and being used as reference vaccine virus against the H5N1 Clade 2. 3. 2 viruses.关键词
H5亚型/Clade2.3.2/禽流感病毒/反向遗传Key words
H5/ Clade2. 3. 2/ avian influenza virus/ reverse genetics分类
农业科技引用本文复制引用
张妍,张文俊,李群辉,刘晓文,刘文博,刘秀梵..Clade2.3.2H5N1亚型禽流感疫苗候选株的构建[J].畜牧兽医学报,2012,43(6):915-921,7.基金项目
现代农业产业技术体系建设专项资金(nycytx-41-G07) （nycytx-41-G07）
国家重点基础研究发展计划(973计划)(2011CB505003) （973计划）
