畜牧兽医学报2012，Vol.43Issue(6)：972-978,7.
基于线粒体12S基因对青海地区细粒棘球蚴种群遗传多态性研究
Genetic Polymorphisms of Echinococcus granulosus in Qinghai Based on the Mitochondrial DNA 12S Gene
摘要
Abstract
The aim of the current study was to investigate the genetic polymorphisms of Echinococcus granulosus obtained from sheep in Qinghai Province, and provide the foundational data for prevention and control of echinococcus disease. The complete mitochondrial DNA 12S genes of 42 isolates(33 were from liver and 9 were from lung) were sequenced, and phylogenetic analyses were conducted using neighbor-joining tree-building methods. There were five haplotypes (Hi to H5) detected among the samples, and H5 was the main type. Phylogenetic analysis supported these observations. The haplotype diversity (H) and nucleotide diversity (Pi) were 0. 418 and 0. 000 66, respectively. It was demonstrated that the genetic homology of these isolates with the 12S gene of E. granulosus Gl genotype(AF297617)was over 99. 86%. All 42 samples were identified as E. granulosus sensu stricto (G1-G3 clusters). In this study,genotypes H1 to H4 has not been identified elsewhere.关键词
细粒棘球蚴/12S基因/遗传多态性/青海Key words
Echinococcus granulosus / 12S gene/ genetic diversity/ Qinghai分类
农业科技引用本文复制引用
延宁,杨德英,郝桂英,古小彬,杨光友,聂华明,蒋忠荣,邓世金,余华,严玉宝,达瓦次仁,谢跃,付彦..基于线粒体12S基因对青海地区细粒棘球蚴种群遗传多态性研究[J].畜牧兽医学报,2012,43(6):972-978,7.基金项目
国家科技支持计划项目(2006BA106B09) （2006BA106B09）
国家质检总局科技计划项目(20091K019) （20091K019）
