畜牧兽医学报2012，Vol.43Issue(6)：979-984,6.
妊娠早期蒙古绵羊子宫内膜enJSRV及IFN-τ基因表达水平的分析
Analysis of the Expression of enJSRV and IFN-τ in Endometrium of Mongolian Sheep in Early Pregnancy
摘要
Abstract
In order to detect the expression of enJSRV and IFN-T in endometrial tissue of Mongolian sheep as well as relationship of the expression of enJSRV and the variety of progesterone content in peripheral blood during early pregnancy. TaqMan Real-time Quantify PCR was performed on the relative expression of enJSRV and IFN-T in uterine endometrium, as well as progesterone content in peripheral blood was detected by electrochemiluminescence analyses. The results demonstrated that enJSRV and IFN-r mRNA were expressed specifically in uterine endometrium of Mongolian sheep. The mRNA expression level of enJSRV was high on Day 12-14, while lower than the former on Day 2-10 and 16-30 during pregnancy. IFN-T mRNA was expressed on Day 12-25 of gestation with the maximum value on Day 14, and IFN-T cannot be detected on Day 30. Electrochemiluminescence results showed the progesterone level was 0. 4 ng · mL-1 on Day 2 and rose afterwards on Day 8 of gestation, then kept at about 8. 3 ng · mL-1 on Day 8-16 and decreased during Day 19-30 with the value 2. 5 ng · mL-1 on Day 30. These results indicated that the expression of enJSRV mRNA in endometrium was highly correlated with changes in peripheral blood progesterone content and IFN-T. Furthermore, enJSRV plays a significant role during placental morphogenesis and reproductive biology.关键词
内源性绵羊肺腺瘤病毒/干扰素-τ/孕酮/相对表达量/实时荧光定量PCR/蒙古绵羊/电化学发光法Key words
enJSRV/ IFN-τ/ progesterone/ relative expression/ real-time PCR/ Mongolian sheep/ electrochemiluminescence分类
农业科技引用本文复制引用
刘丽珍,刘淑英..妊娠早期蒙古绵羊子宫内膜enJSRV及IFN-τ基因表达水平的分析[J].畜牧兽医学报,2012,43(6):979-984,6.基金项目
国家自然科学基金(31160493 （）
30960271) （）
内蒙古科技创新引导基金项目(20101808) （20101808）
教育部博士点基金博导类项目(20111515110008) （20111515110008）
