畜牧兽医学报2012，Vol.43Issue(6)：991-996,6.
低氧对牛体细胞体外增殖的影响
The Effect of Low Oxygen on Proliferation of Bovine Somatic Cells in vitro
摘要
Abstract
In order to study the effect of low oxygen on cell proliferation in vitro by culturing bovine somatic cells under the normoxic (20%) and hypoxic (5%) conditions, three types of bovine donor cells commonly used for nuclear transfer (fetal fibroblasts, fetal oviduct epithelial cells, cumulus granulosa cells) were cultured in 20% and 5% oxygen content of the culture environment for continuous subculture and cell colony formation, at the same time, cell proliferation lifespan (be determined by Population doubling level) and colony formation efficiency. The results indicated that 5% oxygen environment promoted the three kinds of cells proliferation in vitro, and proliferation lifespan of cells in hypoxic group extended significantly (50. 61 ±2. 47, 16. 35±O. 43, 43. 38±0. 84 vs 27. 42±0. 23, 12.14±0. 83, 32. 76±l. 53, P<0. 01). In the case of culture at 500 cells · dish"1 (diameter 100 mm), the cell colony formation efficiency of fetal oviduct epithelial cells cultured in low oxygen was much higher than that cultured in normal oxy-gen ((53. 05±4. 62)% vs (36. 68±5. 68)%, P<0. 01), however, fetal fibroblasts and cumulus granulosa cells in hypoxic group displayed only a litter more cell colonies than the normoxic group, the difference was not significant (P>0. 05). When seeding at one cell · well"1 in 96-well plates, single-cell colony formation efficiency of hypoxic group was significantly higher than that of the normoxia group ( (21. 60±2. 37)%, (22. 29±5. 42)%, (27. 92±3. 69) % vs (12. 01± 1.42)%, (7.92±2.86)%, (10. 49±3. 07)%, P<0. 01 or P<0. 05). The result indicate that reducing the oxygen content(20%) of in vitro culture conditions to low oxygen (5%) , which better approximate to the physiologic situation, can extend cell proliferation lifespan and improve the efficiency of single-cell colony formation, low oxygen has a good role in promoting cell growth.关键词
低氧/牛体细胞/体外增殖Key words
low oxygen/ bovine somatic cells/ proliferation in vitro分类
农业科技引用本文复制引用
李松,朱士恩,丁方荣,王海萍,王莉莉,李京,郑敏,王美利,王超,戴蕴平..低氧对牛体细胞体外增殖的影响[J].畜牧兽医学报,2012,43(6):991-996,6.基金项目
国家转基因生物新品种培育重大专项(2008ZX08007-001) （2008ZX08007-001）
