西南林业大学学报2012，Vol.32Issue(3)：51-54,4.DOI:10.3969/j.issn.2095-1914.2012.03.011
蓖麻LPAAT基因序列的SNPs及与油脂的相关性
Correlation Between Single Nucleotide Polymorphisms of LPAAT Gene Sequence and Oil Content of Ricinus communis
摘要
Abstract
The LPAAT gene of Ricinus communis plays an important role in synthesizing and regulating the fatty acid (triacylglycerols, TAG). In order to study the diversity of LPAAT gene in R. communis, the LPAAT sequencing of the R. communis germplasm collected from 32 different areas was conducted by referring the LPAAT gene sequencing primers of R. communis in the GenBank, and a genomic sequence with 804bp was obtained. The results from the diversity analyses showed that there were 2 SNPs and 3 InDels among the 804bp sequence, the SNP frequency was 1/161 bp, and the nucleotide diversity index ( Pi) was 0.000 67. There were 1 SNP and 2 InDels located at exon, two of them were nonsense mutations; the other one was missense mutalion. The results showed that the exon 10 of LPAAT gene was related to the oil content of R. communis.关键词
蓖麻/单核苷酸多态性/LPAAT/油脂Key words
Ricinus communis/ single nucleotide polymorphism (SNP) / LPAAT/ oil分类
农业科技引用本文复制引用
徐宸敏,刘小烛,刘爱忠..蓖麻LPAAT基因序列的SNPs及与油脂的相关性[J].西南林业大学学报,2012,32(3):51-54,4.基金项目
国家自然科学基金(30871548)项目资助 （30871548）
中国科学院"百人计划"择优支持基金(KSCX2-Y-CW-035-1)项目资助. （KSCX2-Y-CW-035-1）
