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蕈状芽孢杆菌SH-1抗菌活性物质发酵条件优化

孙会刚 黄海亮 郑进 金鑫

徐州工程学院学报:自然科学版2012,Vol.27Issue(2):45-49,5.
徐州工程学院学报:自然科学版2012,Vol.27Issue(2):45-49,5.

蕈状芽孢杆菌SH-1抗菌活性物质发酵条件优化

Optimization of Fermentation Conditions for High Production of Antimicrobial Substance by Bacillus Mycoides SH-1

孙会刚 1黄海亮 1郑进 1金鑫1

作者信息

  • 1. 徐州工程学院,江苏徐州221008
  • 折叠

摘要

Abstract

One strain of Bacillus SH-1 was screened from soil, have been able to produce lots of active antibacterial substance. and was proved through experiment to The strain was identified as Bacillus mycoides SH-1. Through single factor experiment and orthogonal test, the fermentation conditions for Bacillus mycoides SH-1 to produce antibacterial substance was optimized, and the antibacterial activity was tested by Oxford cup method. The best fermentation conditions for Bacillius mycoides SH-1 to produce antibacterial substance was determined as temperature 36 ℃, pH 8, speed 180 r/min, inoculum amount 4%, fermentation time 24 h.

关键词

芽孢杆菌/发酵条件/优化/正交实验/抑菌

Key words

Bacillus/fermentation condition/orthogonal test/antibacterial substance

分类

化学化工

引用本文复制引用

孙会刚,黄海亮,郑进,金鑫..蕈状芽孢杆菌SH-1抗菌活性物质发酵条件优化[J].徐州工程学院学报:自然科学版,2012,27(2):45-49,5.

基金项目

基金项目:河北省自然科学基金项目 ()

徐州工程学院科研项目 ()

徐州工程学院学报:自然科学版

1674-358X

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