烟草科技Issue(4):28-32,5.
Amplex Red/HRP荧光法测定卷烟主流烟气中的过氧化氢
Determination of Hydrogen Peroxide in Mainstream Cigarette Smoke by Highly Selective Fluorescence Method with Amplex Red/HRP
摘要
Abstract
A highly selective fluorescence method with Amplex Red HRP was developed to determine the hydrogen peroxide in mainstream cigarette smoke. This method's LOD=13.5 nmol/L, recovery 98.3%-101.7% and RSD<3.17% (n=5). The level of hydrogen peroxide in mainstream smoke was of micrograms per cigarette and positively correlated with tar delivery. For the cigarettes with the same tar delivery, the delivery of hydrogen peroxide was dependent on their design parameters. This method is suitable for determining the delivery of H2O2 in mainstream cigarette smoke.关键词
过氧化氢/卷烟/主流烟气/Amplex Red/荧光法Key words
Hydrogen peroxide (H2O2)/ Cigarette/ Mainstream smoke/ Amplex Red/ Fluorescence method分类
轻工纺织引用本文复制引用
彭书海,张映,刘海林,庄亚东,蒋承勇,孙世豪,宗永立,谢剑平..Amplex Red/HRP荧光法测定卷烟主流烟气中的过氧化氢[J].烟草科技,2012,(4):28-32,5.基金项目
国家自然科学基金资助项目“自旋捕集/MALDI-FTMS快速分析烟气复杂体系中的气相自由基”(20875102). (20875102)