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Amplex Red/HRP荧光法测定卷烟主流烟气中的过氧化氢

彭书海 张映 刘海林 庄亚东 蒋承勇 孙世豪 宗永立 谢剑平

烟草科技Issue(4):28-32,5.
烟草科技Issue(4):28-32,5.

Amplex Red/HRP荧光法测定卷烟主流烟气中的过氧化氢

Determination of Hydrogen Peroxide in Mainstream Cigarette Smoke by Highly Selective Fluorescence Method with Amplex Red/HRP

彭书海 1张映 2刘海林 1庄亚东 2蒋承勇 1孙世豪 1宗永立 1谢剑平1

作者信息

  • 1. 中国烟草总公司郑州烟草研究院,郑州高新技术产业开发区枫杨街2号 450001
  • 2. 江苏中烟工业有限责任公司技术研发中心,南京市建邺区梦都路30号 210093
  • 折叠

摘要

Abstract

A highly selective fluorescence method with Amplex Red HRP was developed to determine the hydrogen peroxide in mainstream cigarette smoke. This method's LOD=13.5 nmol/L, recovery 98.3%-101.7% and RSD<3.17% (n=5). The level of hydrogen peroxide in mainstream smoke was of micrograms per cigarette and positively correlated with tar delivery. For the cigarettes with the same tar delivery, the delivery of hydrogen peroxide was dependent on their design parameters. This method is suitable for determining the delivery of H2O2 in mainstream cigarette smoke.

关键词

过氧化氢/卷烟/主流烟气/Amplex Red/荧光法

Key words

Hydrogen peroxide (H2O2)/ Cigarette/ Mainstream smoke/ Amplex Red/ Fluorescence method

分类

轻工纺织

引用本文复制引用

彭书海,张映,刘海林,庄亚东,蒋承勇,孙世豪,宗永立,谢剑平..Amplex Red/HRP荧光法测定卷烟主流烟气中的过氧化氢[J].烟草科技,2012,(4):28-32,5.

基金项目

国家自然科学基金资助项目“自旋捕集/MALDI-FTMS快速分析烟气复杂体系中的气相自由基”(20875102). (20875102)

烟草科技

OA北大核心CSTPCD

1002-0861

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