烟草科技Issue(4)：51-55,5.
剑桥滤片-液-液捕集系统各部分烟气捕集物的细胞毒性比较
Comparison on Cytotoxicity of Cigarette Smoke Components Trapped at Each Stage in Cambridge Filter-Solvent-Solvent Systems
摘要
Abstract
In order to investigate the effects of cigarette smoke trapping on in vitro cytotoxicity test, 3 kinds of trapping systems, including Cambridge filter-pII7.0 PBS-acetone, Cambridge filtcr-pH7.2 PBS-ethyl acetate and Cambridge filter-pH7.4 PBS-70% glycerol, were used to collect participate matter and gas phase from the mainstream smoke of the same cigarettes, the cytotoxicity of smoke trapped at each stage in the systems to CHO (Chinese Hamster ovary) cells was tested by neutral red method, and the chemical compositions in the matter trapped by organic solvents were analyzed by gas chromatography-mass spectrometry. The results indicated that: 1) the cytotoxicity of the matter trapped by Cambridge filter was more significant than that by PBS and organic solvent; 2) for the 2nd stage trapped matter, the cytotoxicity of that trapped by pH7.4 PBS was significantly higher than that by pH7.0 and pH7.2; 3) for the 3rd stage trapped matter, the cytotoxicity of mat trapped by the 70% glycerol was significant, that by acetone and ethyl acetate was less and close. The vapor phase components trapped by the 3 organic solvents were mainly aldehydes, esters, alkanes and benzene analogues. There were more alkanes and benzene analogues captured by 70% glycerol. In conclusion, the smoke condensate trapped by Cambridge filter reflected part of neutral red cytotoxicity of smoke to CHO cell, while that trapped by Cambridge filter-pH7.4 PBS-70% glycerol more completely reflected the cytotoxicity of smoke.关键词
烟气捕集/剑桥滤片/磷酸缓冲液/丙酮/乙酸乙酯/甘油/细胞毒性/中性红Key words
Cigarette smoke trapping/ Cambridge filter, Phosphate buffer solution/ Acetone/ Ethyl acetate/ Glycerol/ Cytotoxicity/ Neutral red分类
轻工纺织引用本文复制引用
夭建华,米其利,耿永勤,杨叶昆,黄海涛,李雪梅,缪明明,唐萍..剑桥滤片-液-液捕集系统各部分烟气捕集物的细胞毒性比较[J].烟草科技,2012,(4):51-55,5.基金项目
国家烟草专卖局基金项目“烟用添加剂安全性评价关键技术研究”(110200902060) （110200902060）
云南省应用基础研究项目“烟草添加剂安全性评价的体外毒理学方法研究”(2009ZC190M) （2009ZC190M）
云南省烟草化学创新团队建设项目“云南烟草科学研究院烟草化学省创新团队”0(2009CI014)和云南省中烟工业公司项目“烟草行业烟用添加剂安全性评价体系建设”(2009JC01). （2009CI014）
