医药导报2012，Vol.31Issue(6)：701-705,5.DOI:10.3870/yydb.2012.06.004
白及多糖对氧化损伤的人角质形成细胞JAK/STAT信号通路的影响
Effects of Bletilla Striata Polysaccharide on JAK/STAT Signaling Pathway in Oxidative Damaged Human Keratinocyte
王刚 1常明泉 1杨光义 1杜士明 1叶方 1张秀华 1杨金霞1
作者信息
- 1. 湖北医药学院附属太和医院药学部,湖北十堰442000
- 折叠
摘要
Abstract
Objective To investigate the protective effect and mechanism of Bletilla Striata polysaccharide on JAK/ STAT signaling pathway in human keratinocytes (HKCs) with inflammation induced injury by CoCl2. Methods After pretreated with Bletilla Striatu polysaccharide, HKCs were interfered with certain dosages of CoCl2 to establish an inflammation induced injury cell model by oxidative stress from chemical oxidizing agent. The cell viability was detected by MTT method. The levels of cytokine TNF-α, IL-6 and IL-8 were detected by ELISA, and expression of JAK2 was determined. Results Bletilla Striata polysaccharide could boost the cell viability in a dose-dependent manner. The contents of TNF-α, IL-6 and IL-8 in the group of high or medium dosage of Bletilla Striata polysaccharide were significantly different from those in the control group (P< 0.05). Conclusion The inflammation injury of HKC can be caused by oxidative stress from CoCl2. Btetilla Striata polysaccharide can inhibit release of TNF-α, IL-6 and IL-8 through regulating expression of JAK2 in JAK/STAT signaling pathway, which might be one of the mechanisms of protection.关键词
白及多糖/人角质形成细胞/JAK/STAT信号通路Key words
Bietilla Striata polysaccharide/ Human keratinocytes/ JAK/STAT signaling pathway分类
医药卫生引用本文复制引用
王刚,常明泉,杨光义,杜士明,叶方,张秀华,杨金霞..白及多糖对氧化损伤的人角质形成细胞JAK/STAT信号通路的影响[J].医药导报,2012,31(6):701-705,5.
