针刺研究2012，Vol.37Issue(2)：93-98,6.
电针对炎性痛大鼠痛行为及颈段脊髓γ-氨基丁酸受体亚型基因表达的影响
Effects of Electroacupuncture of “Futu” (LI 18), etc.on Pain Behavior and Expression of GABA Receptor Subunit Genes in Cervical Spinal Cord in Rats with Thyroid Regional Pain
摘要
Abstract
Objective To observe the effect of electroacupuncture (EA) at different acupoints on the expression of gamma aminobutyric (GABA) receptor (R) subunit genes in the cervical spinal cord in rats with thyroid regional inflammatory pain so as to analyze its analgesic mechanism for thyroid surgery. Methods A total of 50 Wlstar rats were randomized into control, model, Futu (LI 18), Hegu(LI 4)-Neiguan(PC 6, LI4-PC6), Zusanli (ST 36)-Yanglingquan (GB34, ST 36-GB 34) groups, with 10 rats in each group. Thyroid regional pain model was established by subcutaneous injection of 2. 5% formalin (100 μL).Ten minutes after modeling, EA (2 Hz/15 Hz. 1 mA) was applied to LI 18, LI 4-PC 6 and ST 36-GB 34 for 30 min, respectively. The animals' face-grooming (FG) times in 5 min and thermal pain threshold (paw withdrawal latency, PWL) were recorded. The expression of GABAA R, GABAB R 1, and GABAs R 2 genes in the cervical 1 - 3 segments of the spinal cord 80 min after modeling was detected by using reversed transcription-polymerase chain reaction (RT-PCR). Histological changes of the tissues of the thyroid region were observed by using HE staining. Results After subcutaneous injection of formalin, the animals' FG times in 5 min were increased considerably and the thermal pain threshold was decreased obviously in the model group (P<0. 05). Concomitantly. the expression levels of GABAgR 1, and GABABR 2 genes of the cervical spinal cord were upregulated significantly in the model group (P<0. 05), and GABAAR expression was increased slightly (P>0. 05). Compared with the model group, the FG times in 5 min in the LI 18 and LI 4-PC 6 groups at 40 min and 70 min after modeling were decreased significantly ( P<0. 05). and their thermal pain threshold values were increased markedly (P<0. 05). The expression levels of GABAa Ft 1 mRNA, GABAB R 2 mRNA and GABAA R mRNA were significantly higher in the LI 18 and LI 4-PC 6 groups than in the model group (P<0. 05). The expression level of GABABR 1 mRNA of the ST 36-GB 34 group was obviously higher than that of the model group (P<0. 05). The inflammatory cells in the formalin-injected thyroid region were relatively fewer in the LI 18 and LI 4-PC 6 groups than in the model group. Conclusion Both EA of LI 18 and LI 4-PC 6 can significantly suppress formalin-injection induced pain reactions in the thyroid region, which may be closely associated with its effects in upregulating expression levels of cervico-spinal GABABR 1 mRNA, GABABR 2 mRNA and GABAAR mRNA, and reduce regional inflammatory reactions in the thyroid region.关键词
甲状腺区炎性反应/电针镇痛/颈段脊髓/γ-氨基丁酸受体亚型Key words
Cervical inflammatory pain/ Electroacupuncture analgesia/ Spinal cord/ GABABR 1 mRNA/ GABABR 2 mRNA/ GABAAR mRNA分类
医药卫生引用本文复制引用
高永辉,陈淑萍,王俊英,乔丽娜,韩焱晶,林丹,吉长福,徐秋玲,刘俊岭..电针对炎性痛大鼠痛行为及颈段脊髓γ-氨基丁酸受体亚型基因表达的影响[J].针刺研究,2012,37(2):93-98,6.基金项目
项目来源:国家“973”计划中医理论研究专项(No.2007 CB 512505)、国家自然科学基金项目(No.30973796、No.30973796、No.81102647)、国家自然科学基金重大项目(No.90709031) （No.2007 CB 512505）
