中国病理生理杂志2012，Vol.28Issue(7)：1160-1165,6.DOI:10.3969/j.issn.1000-4718.2012.07.002
14-3-3γ对烧伤及LPS诱导所致大鼠心肌损伤的保护作用
Protective role of 14-3-3γ in burn and LPS-induced rat myocardial injury
摘要
Abstract
ADM: To study the protective role of 14 - 3 - 3γ in burn or LPS - induced myocardial injury. METHODS: The rat model of burn or LPS - induced injury was established. The heart functions and 14 - 3 - 3γ protein expression were detected 3 h, 6 h, 12 h and 24 h after treatment. Primary neonatal rat cardiomyocytes were used in vitro. pFLAG — 14—3—3γ plasmid was constructed and transfected into the cardiomyocytes 24 h before LPS — induced injury. The injury in the cardiomyocytes was evaluated by measuring the cell viability and the level of lactate dehydrogenase ( LDH ). Apoptosis of cardiomyocytes was detected by flow cytometry. Opening of mitochondrial permeability transition pore ( mPTP ) was also determined by Ca 2+ — induced swelling of isolated myocardial mitochondria. RESULTS: The expression of 14 — 3 — 3γ was elevated following the burn or LPS — induced myocardial injury in vivo. In vitro, transfection with pFLAG - 14 - 3 - 3γ plasmid in to the cardiomyocytes significantly protected against LPS - induced injury. Compared with the cardiomyocytes without transfection with pFLAG — 14—3 — 3γ plasmid, higher cell viability rate and lower LDH release, cell apoptosis and mPTP opening were observed in the cardiomyocytes transfected with pFLAG — 14 — 3 — 3γ plasmid. CONCLUSION: The 14 -3 -3γ protein protects the heart against burn or LPS -induced injury by inhibiting the mPTP opening.关键词
14-3-3γ蛋白/脂多糖类/烧伤/心肌/线粒体通透性转换孔Key words
14-3-3γ protein/ Lipopolysaccharides/ Burns/ Myocardium/ Mitochondrial permeability transition pore分类
医药卫生引用本文复制引用
刘丹,尹东,严广华,孙惦,许旻 ,何明..14-3-3γ对烧伤及LPS诱导所致大鼠心肌损伤的保护作用[J].中国病理生理杂志,2012,28(7):1160-1165,6.基金项目
国家自然科学基金资助项目(No.30460048 （）
No.81072632 （）
No.81160402 （）
No.81100104) （）
博士后基金资助项目(No.20110491496) （No.20110491496）
