中国病理生理杂志2012，Vol.28Issue(7)：1166-1171,6.DOI:10.3969/j.issn.1000-4718.2012.07.003
血管紧张素Ⅱ升高血管内皮细胞中ROS水平并激活自噬通路
AngiotensinⅡ activates autophagy pathway through elevating ROS level in vascular endothelial cells
摘要
Abstract
AIM: To evaluate the effects of angiotensin II ( Ang II ) on autophagy induction in vascular endo-thelial cells. METHODS: Human vascular endothelial EA. hy926 cells were used in the study. Intracellular reactive oxygen species ( ROS ) levels were detected by a microplate reader after the cells were treated with Ang II ( 10 - mol/L ) or Ang II combined with antioxidant N — acetyl — L — cysteine ( NAC, 50 μmol/L ) for 24 h. The protein levels of LC3 — II was detected by Western blotting after the cells were stimulated by different concentrations ( 10-8, 10-7 , 10-6 mol/L) of Ang II for 24 h or by Ang II ( 10 -7 mol/L ) for different time (Oh, 6 h, 12 h, 24 h, 36 h ). The number of autophago-somes was evaluated by fluorescence microscopy after stained with acridine orange. Similarly, the protein level of LC3 - II and the number of autophagosomes were detected after treated with Ang II( 10-7mol/L), Ang II combined with autophagy inhibitor 3 - methyladenine ( 3 - MA ) at concentration of 2 mmol/L or Ang II combined with NAC at concentration of 50 μmol/L. RESULTS: Intracellular ROS level and LC3 - II protein level were significantly increased ( P < 0. 05 ) after the cells were treated with Ang II , accompanied by the significant increase in the number of autophagosomes. Ang II — induced autophagy ( as showed both in LC3 — II protein level and autophagosomes ) was dramatically down — regulated by the treatment with 3 - MA or NAC in EA. hy926 cells ( P <0. 05 ). CONCLUSION: Ang I induces autophagy through elevating ROS levels in EA. hy926 cells.关键词
自噬/血管紧张素Ⅱ/血管内皮细胞/活性氧簇/氧化性应激Key words
Autophagy/ Angiotensin II / Vascular endothelial cells/ Reactive oxygen species/ Oxidative stress分类
医药卫生引用本文复制引用
范俊,杨成明,连继勤,曾春雨,倪振洪,林德胜,冉希..血管紧张素Ⅱ升高血管内皮细胞中ROS水平并激活自噬通路[J].中国病理生理杂志,2012,28(7):1166-1171,6.基金项目
国家自然科学基金资助项目(No.30371363) （No.30371363）
