摘要
Abstract
ADM: To study the effect of miR - 18α on angiogenesis of human aortic artery endothelial cells. METHODS: After 10 nmol/L miR - 18α mimics or 20 nmol/L inhibitor was transfected into human aortic endothelial cells, the expression level of miR - 18α was determined by qRT - PCR, and the capacities of endothelial cell angiogenesis, such as migration, adhesion and tube formation, were observed. RESULTS: Forty - eight hours after transfection with miR — 18α mimics, the expression of miR — 18α was as 608 folds as the control ( P <0. 05 ), but decreased to 31 % of the control level in miR — 18α inhibitor transfection group ( P <0. 05 ). Compared with control group, the numbers of endothelial cells, which migrated to the lower Transwell chamber and formed capillary — like tubes, declined by 60% and 52% , respectively, in miR — 18α mimics group ( P <0. 01 ), and they increased by 100% and 84% , respectively, in miR — 18α inhibitor transfection group ( P < 0.05, P < 0.01 ). In addition, the inhibitor treatment group displayed more potent adhesion capacity, 43% higher than that in control group ( P <0. 05 ). CONCLUSION: miR - 18α is involved in angiogenesis of human arterial endothelial cells, and might be a potential molecular therapeutic target of vascular diseases.
关键词
miR-18a/人主动脉内皮细胞/血管生成Key words
miR - 18α/ Human aortic endothelial cells/ Angiogenesis分类
医药卫生
