中国病理生理杂志2012，Vol.28Issue(7)：1192-1196,5.DOI:10.3969/j.issn.1000-4718.2012.07.008
二苯乙烯苷对LPC损伤血管内皮细胞的保护作用及其机制
2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside protects against LPC-induced endothelial cell injury
摘要
Abstract
AIM: To observe the protective effect of 2,3 ,5,4' — tetrahydroxystilbene — 2 — 0 — (3 — D— glucoside ( TSG ) on lysophosphatidylcholine ( LPC ) - induced vascular endothelial cell injury. METHODS: The 3rd and 4th generations of human umbilical vein endothelial cells ( HUVECs ) were cultured in vitro and propagated. The cells were randomly divided into 3 groups: control group, model group ( LPC ) and experimental group ( TSG + LPC ). The cells in control group were not treated with any reagent. To establish endothelial cell injury model, LPC was administered to HUVECs at concentration of 10 mg/L and incubated with the cells for 24 h. In TSG + LPC group, TSG was administered to HUVECs at concentrations of 10.0, 1.0 and 0. 1 μmol/L 1 h before administration of LPC, and then the cells were incubated for 24 h. The cell viability, the content of asymmetric dimethyl arginine ( ADMA ) and NO, and apoptotic rate were detected. RESULTS: Compared with control group, ADMA content in the cell culture supernatants and apoptotic rate of the HUVECs in LPC group were significantly increased, while the NO content and cell viability were notably decreased. Compared with LPC group, ADMA content and apoptotic rate in TSG + LPC group was significantly decreased, while the NO content and cell viability were notably increased. CONCLUSION: TSG may protect LPC — injured vascular endothelial cells by attenuating the expression of ADMA and enhancing the production of NO, thus inhibiting apoptosis and increasing cell survival rate.关键词
2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷/内皮细胞/溶血磷脂酰胆碱/非对称性二甲基精氨酸/一氧化氮/细胞凋亡分类
医药卫生引用本文复制引用
张燕堂,张颖,郑文,杨华,王家富,商战平..二苯乙烯苷对LPC损伤血管内皮细胞的保护作用及其机制[J].中国病理生理杂志,2012,28(7):1192-1196,5.基金项目
泰山医学院青年基金资助项目(No.2010ZRQN046) （No.2010ZRQN046）
