中国病理生理杂志2012，Vol.28Issue(7)：1247-1252,6.DOI:10.3969/j.issn.1000-4718.2012.07.018
光敏化促进姜黄素诱导人胃癌MGC-803细胞凋亡
Photoactivation promotes curcumin to induce apoptosis of human gastric cancer MGC-803 cells
摘要
Abstract
AIM: To investigate the effect of photoactivated curcumin on apoptosis of human gastric cancer MGC — 803 cells. METHODS: The effect of photoactivated curcumin on the growth inhibitory rate of gastric cancer MGC - 803 cells was detected by MTT assay. The changes of nuclear morphology were observed under optical microscope with Hoechst 33258 fluorescent staining. The apoptotic rate, mitochondrial membrane potential, intracellular reactive oxygen species and Ca2 + level was determined by flow cytometry. The activity of caspase - 3 , caspase - 8 and caspase - 9 was detected by colorimetry. The protein levels of cytochrome C, Bcl - 2, Bax and heat - shock protein 70 ( HSP70 ) were analyzed by Western blotting. RESULTS: The growth inhibitory rate of MGC - 803 cells treated with curcumin at concentration of 5. 0 μmol/L was ( 29.74 ± 2. 30 )% . Some apoptotic cells were observed under optical microscope, and the apoptotic rate was ( 12. 54 ± 1. 75 )%. The growth inhibitory rate of MGC — 803 cells treated with photoactivated curcumin was ( 44. 93 ±3. 61 )%. Significant morphological changes in the nucleus, such as chromatin condensation and apoptotic body formation, were observed under light microscope, and the apoptotic rate was ( 26. 58 ±2. 67 )% . The cell cycle was arrested at G0/G1 phase. Compared with curcumin group, significant reduction in mitochondrial membrane potential,significant increase in cytochrome C, intracellular reactive oxygen species, Ca2 + level and the activity of caspase — 3, caspase — 8 and caspase —9 were observed in photoactivated curcumin group ( P <0. 01 ). Photoactivated curcumin also significantly inhibited the protein expression of Bcl -2 and HSP70 in the cells. CONCLUSION: Photoactivated curcumin enhances the apoptosis of gastric cancer MGC - 803 cells by inhibiting Bcl - 2 expression and promoting the mitochondrial pathway.关键词
姜黄素/光敏化/MGC-803细胞/细胞凋亡Key words
Curcumin/ Photoactivation/ MGC -803 cells/ Apoptosis分类
医药卫生引用本文复制引用
旷焱平,陈垦,何博华,王林静,祝爱珍,刘成成,刘革修..光敏化促进姜黄素诱导人胃癌MGC-803细胞凋亡[J].中国病理生理杂志,2012,28(7):1247-1252,6.基金项目
广东省中医药管理局项目(No.2010414) （No.2010414）
广东药学院护理学院广东省特色专业建设经费资助项目(No.TS11060) （No.TS11060）
