中国病理生理杂志2012，Vol.28Issue(7)：1258-1261,4.DOI:10.3969/j.issn.1000-4718.2012.07.020
PMA特异性抑制K562细胞BCR/ABL和Fyn mRNA的表达
PMA specifically inhibits mRNA expression of BCR/ABL and Fyn in K562 cells
摘要
Abstract
ADM: To study the effects of phorbol 12 — myristate 13 — acetate ( PMA ) on the mRNA expression of BCR/ABL and Fyn in K562 cells, and to explore their relationship. METHODS: The K562 cells were stimulated by PMA at a series of concentrations ( 1 ~ 250 μg/L ) for 24 h, and the mRNA expression levels of BCR/ABL and Fyn in K562 cells were detected by real - time fluorescence quantitative PCR . The relative changes of both mRNA expression were measured using 2 -ΔΔCt formula. RESULTS: PMA significantly inhibits the mRNA levels of BCR/ABL and Fyn in a dose - dependent manner, and the correlation of these inhibitory effects were significant. Compared with gene Molt - 4 cells, the inhibition by PMA was specific for K562 cells. The K562 cells were induced to differentiate to be pseudopodium -like ceUs. CONCLUSION: The PMA downregulates the mRNA level of Fyn by inhibiting BCR/ABL fusion gene.关键词
12-肉豆蔻酸-13-乙酸佛波酯/Fyn蛋白/融合蛋白,BCR-ABLKey words
Phorbol 12 - myristate 13 - acetate/ Fyn protein/ Fusion proteins, BCR - ABL分类
医药卫生引用本文复制引用
王冠明,陈小华,林晨,陈少华,杨力建,王鹏程,李扬秋..PMA特异性抑制K562细胞BCR/ABL和Fyn mRNA的表达[J].中国病理生理杂志,2012,28(7):1258-1261,4.基金项目
广东省自然科学基金资助项目(No.06025169) （No.06025169）
广州市科技支撑计划项目(No.2009Z1-E161) （No.2009Z1-E161）
广东省自然科学基金博士科研启动项目(No.10451063201005505) （No.10451063201005505）
广东省卫生厅医学科研基金资助项目(No.A2010313) （No.A2010313）
