中国病理生理杂志2012，Vol.28Issue(7)：1262-1268,7.DOI:10.3969/j.issn.1000-4718.2012.07.021
DNMT在胃癌中的表达及DNMT抑制剂5-氮杂-2'-脱氧胞苷对胃癌的影响
Expression of DNMT in gastric cancer and effects of DNMT inhibitor 5-aza-2-deoxycytidine on gastric cancer
何苗 1汤为学 2姜蓉 3范晶 4张能 1查朗 1王子卫1
作者信息
- 1. 重庆医科大学附属第一医院普通外科,重庆,400016
- 2. 重庆医科大学附属第一医院实验研究中心,重庆,400016
- 3. 重庆医科大学基础医学院,重庆,400016
- 4. 重庆医科大学附属第一医院急诊科,重庆,400016
- 折叠
摘要
Abstract
ADM: To investigate the protein expression of DNA methyltransferases ( DNMT1, DNMT2, DN-MT3 A, DNMT3 B and DNMT3 L ) in gastric cancer and the effects of DNMT inhibitor ( 5 — aza — 2' — deoxycytidine ) on gastric cancer. METHODS: The method of immunohistochemistry was used to detect DNMT expression in 60 pairs of gastric cancer and corresponding non - cancerous tissues. The expression of DNMT in gastric cancer cells ( SGC -7901 and MKN — 45 ) and gastric mucosa cells ( GES — 1 ) was detected by immunofluorescence and Western blotting. The proliferation, cell cycle and apoptosis in the cell lines of SGC —7901, MKN —45 and GES — 1 were determined by MTT assay and flow cytometry with 5 - aza - 2' - deoxycytidine treatment. RESULTS: Both gastric cancer and corresponding non - cancerous tissues expressed 5 kinds of DNMT proteins, but the expression levels of DNMT1 and DNMT3A were significantly lower in gastric cancer than those in non — cancerous tissues ( P <0.01 ). The expression levels of DNMT2 and DNMT3L were also lower in the cancer than those in non - cancerous tissues ( P <0. 05 ). No difference of DNMT3B expression level between cancer and non - cancerous tissues was observed ( P > 0.05 ). On the other hand, the cells lines of SGC - 7901, MKN -45 and GES — 1 also expressed DNMT proteins. Except DNMT3B, no difference of the other kinds of DNMT between gastric cancer cells and gastric mucosa cells was observed. DNMT inhibitor 5 — aza — 2' — deoxycytidine did not have any effect on the growth rate, cell cycle distribution or apoptotic rate in gastric cancer cells and gastric mucosa cells. CONCLUSION : Gastric cancer expresses low levels of DNMT proteins than normal gastric mucosa. 5 — Aza — 2' — deoxycytidine is not useful for treating gastric cancer.
关键词
DNA甲基化转移酶/胃肿瘤/5-氮杂-2'-脱氧胞苷Key words
DNA methyltransferase/ Stomach neoplasms/ 5 - Aza - 2' - deoxycytidine分类
生物科学引用本文复制引用
何苗,汤为学,姜蓉,范晶,张能,查朗,王子卫..DNMT在胃癌中的表达及DNMT抑制剂5-氮杂-2'-脱氧胞苷对胃癌的影响[J].中国病理生理杂志,2012,28(7):1262-1268,7.
