中国动脉硬化杂志2012，Vol.20Issue(4)：289-294,6.
槟榔碱对泡沫细胞胆固醇流出和ABCA1表达的影响
Effect of Arecoline on Cholesterol Efflux and ATP-Binding Cassette Transporter A1 Expression in RAW 264.7 Macrophage-Derived Foam Cells
摘要
Abstract
Aim To explore the effect of arecoline (Are) on the formation of foam cells induced by the oxidized low density lipoprotein (ox-LDL) and analyze the possible mechanisms. Methods RAW 264. 7 macrophages were incubated by ox-LDL (50 mg/L) for 48 h to induce foam cells. Arecoline (10~'mol/L, 10"5mol/L and 10~4mol/L) was added for 48 h at the same time. The cellular lipid accumulation was examined by oil red staining. The cellular content* of total cholesterol ( TC) and free cholesterol (FC) were detected by high performance liquid chromatography assays. Cholesterol efflux from macrophages was examined by 3H labed cholesterol. The expressions of ATP-binding cassette transporter Al (ABCA1) were detected by Real-time PCR and Western blot. Results Oil red-staining positive cells were found and macrophages were filled with lipid droplet in foam cells model group. Compared with the foam cells, arecoline (10 "! and 10 '* mol/L) significantly decreased the amount of oil red-staining positive cells and the contents of lipid droplet of foam cells. Compared with thecontrol macrophages group, the contents of TC, FC, cholesteryl ester (CE) and CE/TC ratio in model group were significantly increased in foam cells model group. Arecoline (10~s and 10 4mol/L)decreased the contents of TC, FC, cholesteryl ester (CE) and CE/TC ratio, and increased significantly cholesterol efflux and expression of ABCA1 compared with foam cells model group. Conclusion Arecoline inhibits the formation of foam cell induced by ox-LDL and up-regulates of expression ABCA1 in RAW 264.7 macrophage-derived foam cells.关键词
槟榔碱/泡沫细胞/胆固醇流出/ABCA1/动脉粥样硬化Key words
Arecoline/Foam Cells/Cholesterol Efflux/ATP-Binding Cassette Transporter Al/Atherosclerosis分类
医药卫生引用本文复制引用
欧阳新平,周寿红,田绍文,李兴,何平平,尹蔚兰,周钰娟,唐朝克..槟榔碱对泡沫细胞胆固醇流出和ABCA1表达的影响[J].中国动脉硬化杂志,2012,20(4):289-294,6.基金项目
国家自然科学基金项目(81070220) （81070220）
