中国动脉硬化杂志2012，Vol.20Issue(6)：494-498,5.
cAMP反应元件结合蛋白在高糖所致内皮细胞损伤中的作用
Role of cAMP Response Element Binding Protein in Endothelial Cells Injury Induced by High Glucose
摘要
Abstract
Aim To investigate the role of cAMP response element binding protein (CREB) in endothelial cells injury induced by high glucose. Methods The endothelial cell injury model was induced by high glucose. ECV304 cells were divided into six groups: control group, high glucose group, PCI transfection group, PCI transfection + high glucose group, PCI-CREB transfection group and PCI-CREB transfection + high glucose group. The cell survival rate of ECV304 cells was detected by MTT method. Flow cytometry was used to detect the apoptosis rate. The MDA concentration was observed by TBA method and SOD activity was tested by xanthine oxidase method. Results Compared with control group, cell survival rate, SOD activity, MDA concentration and apoptosis rate in PCI-CREB transfection group and PCI transfection group were not significantly different (P > 0. 05). Compared with control group, both cell survival rate and SOD activity in high glucose group showed a significant decrease, however, apoptosis rate and MDA concentration increased significantly (P<0.05). Cell survival rate, SOD activity, MDA concentration and apoptosis rate in PCI transfection + high glucose group was not significantly different compared with high glucose group (P < 0. 05). Compared with high glucose group, both cell survival rate and SOD activity in PCI-CREB iransfection + high glucose group showed a significant increase, however, apoptosis rate and MDA concentration decreased significantly (P<0. 05). Conclusion Overexpression of CREB may protect endothelial cells from high glucose injury.关键词
cAMP反应元件结合蛋白/高糖/内皮细胞/细胞凋亡Key words
cAMP Response Element Binding Protein/High Glucose/Endothelial Cell/Cell Apoplosis分类
医药卫生引用本文复制引用
屈顺林,范文静,张弛,郭芳,罗其富,姜志胜..cAMP反应元件结合蛋白在高糖所致内皮细胞损伤中的作用[J].中国动脉硬化杂志,2012,20(6):494-498,5.基金项目
国家自然基金项目(81100212/H0215)、中国博士点基金新教师类联合项目(20114324120004)、中国博士后科学基金项目(2012M511383)、湖南省教育厅科研项目(11C1094和08c743)和南华大学高层次人才科研启动项目(2011XQD24)资助 （81100212/H0215）
