中国动脉硬化杂志2012，Vol.20Issue(6)：503-507,5.
早期生长反应因子1特异脱氧核酶对大鼠颈动脉损伤后纤溶酶原激活物抑制剂1表达的影响
Effects of DNA Enzyme Targeting Early Growth Response Factor-1 on The Expression of Plasminogen Activator Inhibitor Type-1 after Balloon Injury of Rat Common Carotid Artery
摘要
Abstract
Aim To investigate the effects of DNA enzyme targeting early growth response factor-1 (Egr-1) mR-NA (ED5) on the expression of PAI-1 after vascular balloon injury and the mechanism of inhibiting neointimal hyperplasia. Methods To establish the intimal injury model of left carotid artery in rats. Ninty-six rats were randomly divided into four groups, sham group, MgCl2 group, FuGENE6 group and ED5 group. Six rats were killed at 3 d, 7 d, 14 d and 21 d after operation. Vascular sections were stained with heamotoxylin and eosin to identify pathological changes of vascular by light microscope. Inununohistochemistry stain was used to test the expression of Egr-1 inartenal wall. ELISA kit was used to test the expression of PAI-1 gene in plasma. Results The levels of Egr-1 were reduced after treatment with ED5, and there were significant differences between the ED5 group and the MgCl2 and F11GENE6 group (P < 0. 01). The expression of PAI-1 reduced at every time point in ED5 group (P < 0. 01). Compared with MgCl2 group and F11GENE6 group, the intimal and media thickness and the stenosis rate of vessel in ED5 group at every time points after operation was significantly lowered (P <0.01). Conclusion The level of PAI-1 increase after balloon injury. EDS can throughinhibiting the Egr-I, reduce the expression of PAI-1, and then inhibit early thrombosis and neointimal hyperplasiao关键词
基因转染/早期生长反应因子1/再狭窄/纤溶酶原激活物抑制剂1/脱氧核酶Key words
Gene Transfection/Early Growth Response Factor-1/Restenosis/Plasminogen Activator Inhibitor Type-1/Deoxyribozyme分类
医药卫生引用本文复制引用
张希,刘闺男,王泰然,徐健..早期生长反应因子1特异脱氧核酶对大鼠颈动脉损伤后纤溶酶原激活物抑制剂1表达的影响[J].中国动脉硬化杂志,2012,20(6):503-507,5.基金项目
国家自然科学基金项目(30871074) （30871074）
