中国动脉硬化杂志2012，Vol.20Issue(6)：514-518,5.
川芎嗪减轻大鼠心肌缺血再灌注损伤炎症反应及机制
The Effects of Ligustrazine on Attenuating Inflammatory Reaction of Myocardial Ischemia Reperfusion Injury in Rats and Its Mechanism
摘要
Abstract
Aim To observe the effect of ligustrazine (tetramethylpyrazine.TMP) pretreatment on the inflammatory reaction of myocardial ischemia reperfusion (IR) injury in rats and to investigate the possible mechanism. Methods Forty-eight Sprague-Dawley rats were randomly divided into sham group, IR group, TMP pretreated group, Nc-nitro-L-arginine methyl ester (L-NAME) group and TMP + L-NAME group. All hearts except those in the sham group were subjected to 35 min ischemia followed by 120 min reperfusion. The myocardial structure was observed under microscope. The activity of myocardial superoxide dismutase (SOD) and the content of malondialdehyde ( MDA) , myeloperoxidase (MPO) , interleukin-1 (3(IL-1(3) and nitric oxide (NO) were measured. The expression of endothelial nitric oxide synthase (eNOS) was detected by reverse transcription polymerase chain reaction and Western blot. Results Compared with the IR group, the myocardial tissues of the TMP group showed an increase in the SOD activity, a decrease in the MDA content, the MPO activity and the level of IL-13- TMP pretreatment also increased the NO level and alleviated the neu-trophil infiltration. The expression of eNOS mRNA and protein in the myocardium of rats in the TMP group increased significantly compared with that in the IR group. However, these effects could be significantly reversed by L-NAME which abolished the increase of NO production and eNOS mRNA and protein expression brought by TMP. Conclusions Pretreatment of TMP attenuated the inflammatory reaction of myocardial IR in rats. The cardioprotective mechanism of TMP may relate to its effects of increasing the expression of eNOS and the level of NO.关键词
川芎嗪/缺血再灌注损伤/一氧化氮/内皮型一氧化氮合酶Key words
Ligustrazine/Ischemia Reperfusion Injury/Nitric Oxide/Edothelial Nitric Oxide Synthase分类
医药卫生引用本文复制引用
吕磊,孟庆欣,徐军,宫剑滨,承燕,江时森..川芎嗪减轻大鼠心肌缺血再灌注损伤炎症反应及机制[J].中国动脉硬化杂志,2012,20(6):514-518,5.基金项目
南京军区"十一五"重点课题(06Z38),南京军区南京总医院科研基金(2011017) （06Z38）
