中国骨伤2012，Vol.25Issue(4)：324-327,4.DOI:10.3969/j.issn.1003-0034.2012.04.016
体外肾系膜细胞对成骨细胞增殖及功能的影响
Effects of mesangial cell on proliferation and function of osteoblast in vitro
摘要
Abstract
Objective :To explore the effects and mechanism of mesangial cell (MC) on proliferation and function of osteoblast in vitro from growth factors. Methods: Osteoblast cultured in vitro were divided into normal blood serum group and MC group and cultured supernatant fluid by 10% serum culture medium and 20% MC culture medium. MTT was used to checked the proliferation of osteoblast at the end of 24,48,72,120 h; Expression of BGP and OPN were tested 72 h and 144 h after culture; Expression of IGF-a and TGF-|3 were tested 144 h after culture. Results :The proliferation of osteoblast in MC group was obviously higher than normal blood serum group in different times (P<0.05) ;The level of BGP and OPN of osteoblast was obviously higher than normal blood serum group (11.3%, 16.4%, 55.0% and 39.6%); and the level of IGF-a and TGF-P of osteoblast was obviously higher than normal blood serum group (10.1% and 47.7%). Conclusion: MC can directly act on osteoblast, promote the proliferation and function of osteoblast by promoting secretion of TGF-β关键词
肾小球系膜细胞/成骨细胞/细胞培养技术/细胞增殖Key words
Mesangial cell/Osteoblasts/Cell cultural techniques/Cell proliferation引用本文复制引用
吴锋,林日阳,何立群..体外肾系膜细胞对成骨细胞增殖及功能的影响[J].中国骨伤,2012,25(4):324-327,4.基金项目
十一五国家科技支撑项目(编号:2006BAI04A07) （编号:2006BAI04A07）
上海市科委基础研究重点项目(编号:09JC1413700) （编号:09JC1413700）
上海市教育委员会E-研究院建设计划(NO:E03008) （NO:E03008）
上海市高校创新团队建设项目 （）
