中国计量学院学报2012，Vol.23Issue(2)：182-186,5.
外来入侵生物福寿螺β-actin基因片段的克隆及表达分析
Cloning and expression analysis of cDNA fragments of β-actin protein from the golden apple snail, Pomacea canaliculata
摘要
Abstract
Two cDNA fragment of β-actin gene in Pomacea canaliculata were amplified with a pair of degenerated digonudeotide primers by RT-PCR,and the length was 840 bp encoding 280 amino acids. Blast searching in NCBI showed that the deduced amino acids sequence was highly similar to that of other known related species. A phylogenetic tree analysis based on NJ (Neighbor-Joining) is shown that P. canaliculata belongs to mollusca. In addition, relative expression of β-actin mRNAs in the gill, digestive gland, kidney and foot muscle of snail are keeping constant by semi-quantitative RT-PCR.关键词
福寿螺/β-肌动蛋白/基因克隆/序列分析Key words
Pomacea canaliculata / β-actin/ gene cloning/ sequence analysis分类
生物科学引用本文复制引用
郑国湾,董胜张,俞晓平..外来入侵生物福寿螺β-actin基因片段的克隆及表达分析[J].中国计量学院学报,2012,23(2):182-186,5.基金项目
浙江省科技厅重大科技专项(No.2006C12120). （No.2006C12120）
